# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3706052 | B | Inhibition Assay: A renin substrate of synthetic peptide (Nma-KHPFH LVIHK(Dnp)-NH2) and test compound were mixed, and fluorescence intensity was assayed using a fluorophotometer before starting an enzymatic reaction (exciting wavelength: 340 nm, measuring wavelength: 460 nm). Recombinant human renin was added and the mixture was incubated at 37 C. for an hour, and the fluorescence intensity was measured after the reaction using using a fluorophotometer (exciting wavelength: 340 nm, measuring wavelength: 460 nm). Renin activity was evaluated on the ground of fluorescence intensity which was obtained by deduction of the intensity before the reaction from the intensity after the reaction, and 50% inhibitory concentration (IC50) was calculated from renin activities under the existence of various concentration of the tested compound. | Homo sapiens | 2 | single protein format | BindingDB Database | ||
2. | ALA5149787 | B | Inhibition of human recombinant renin using Nma-Lys-His-Pro-Phe-His-Leu-Val-Ile-His-Lys-Dnp substrate incubated for 1 hr by FRET | Homo sapiens | 23 | single protein format | Scientific Literature | ||
3. | ALA5149788 | B | Inhibition of renin activity in human plasma by competitive radio immunoassay | Homo sapiens | 16 | cell-free format | Scientific Literature |