#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706271	B	Inhibition Assay: Inhibition of kinase activity of Mnk1 and Mnk2a was assessed with the same assay system, using pre-activated GST-Mnk1 or GST-Mnk2a, respectively. The kinase reaction contains 30 uM substrate peptide, 20 uM ATP, 60 nM ligand and one of either 25 nM pre-activated Mnk1 or 2.5 nM pre-activated Mnk2a. The reaction buffer conditions are 16 mM HEPES/KOH pH 7.4, 8 mM MgCl2, 0.4 mM DTT, 0.08% (w/v) bovine serum albumin (BSA, Sigma, Germany, cat. no. A3059), 0.008% (w/v) Pluronic F127 (Sigma, Germany, cat. no. P2443), 3% (v/v) DMSO (Applichem, Germany, cat. no. A3006). The kinase reaction is at 30 C. for 40 min. The kinase reaction is terminated by addition of 0.67 reaction volumes of 1 uM antibody in 20 mM HEPES/KOH pH 7.4, 50 mM ethylenediaminetetraacetic acid, disodium salt (EDTA, Sigma, Germany, cat. no. E5134), 0.5 mM DTT, 0.05% (w/v) polyoxyethylene-sorbitan monolaureate (Tween 20, Sigma, Germany, cat. no. P7949).	Homo sapiens	165	ALA3638571	single protein format	BindingDB Database
2.	ALA4384658	B	Inhibition of MNK in mouse 3T3L1 cells assessed as reduction of eIF4E phosphorylation at 3 uM after 2 hrs by Western blot analysis	Mus musculus	28	ALA4382255	cell-based format	Scientific Literature
3.	ALA4384659	B	Inhibition of MNK in mouse 3T3L1 cells assessed as reduction of eIF4E phosphorylation at 0.1 uM after 2 hrs by Western blot analysis	Mus musculus	6	ALA4382255	cell-based format	Scientific Literature
4.	ALA4384660	B	Inhibition of mTORC2 in mouse 3T3L1 cells assessed as reduction in PKB phosphorylation at Ser473 residue at 0.1 to 5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	cell-based format	Scientific Literature
5.	ALA4384661	B	Inhibition of mTORC1 in mouse 3T3L1 cells assessed as reduction in rpS6 phosphorylation at Ser240/244 residues at 0.1 to 5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	cell-based format	Scientific Literature
6.	ALA4384662	B	Inhibition of mTORC1 in mouse 3T3L1 cells assessed as reduction in 4EBP1 at Ser65 residue phosphorylation at 0.1 to 5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	cell-based format	Scientific Literature
7.	ALA4384663	B	Inhibition of ERK1/2 phosphorylation at Thr202/Tyr204 residues in mouse 3T3L1 cells at 0.1 to 5 uM after 2 hrs by Western blot analysis	Mus musculus	1	ALA4382255	cell-based format	Scientific Literature
8.	ALA4384664	B	Inhibition of ERK signalling in mouse 3T3L1 cells assessed as reduction in RSK phosphorylation at Ser308 residue at 0.1 to 5 uM after 2 hrs by Western blot analysis	Mus musculus	1	ALA4382255	cell-based format	Scientific Literature
9.	ALA4384665	B	Inhibition of MNK1 in C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	single protein format	Scientific Literature
10.	ALA4384666	B	Inhibition of MNK2 in C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	single protein format	Scientific Literature
11.	ALA4384667	B	Inhibition of MNK1 in C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 0.1 to 0.3 uM after 2 hrs by Western blot analysis relative to control	Mus musculus	2	ALA4382255	single protein format	Scientific Literature
12.	ALA4384668	B	Inhibition of MNK2 in C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 0.1 to 0.3 uM after 2 hrs by Western blot analysis relative to control	Mus musculus	2	ALA4382255	single protein format	Scientific Literature
13.	ALA4384669	B	Inhibition of MNK1 in MNK2-knockout C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 0.3 to 5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	single protein format	Scientific Literature
14.	ALA4384670	B	Inhibition of GST-fused MNK1a (unknown origin) expressed in HEK293 cells using eIF4E as substrate after 30 mins by Western blot analysis	Homo sapiens	2	ALA4382255	cell-based format	Scientific Literature
15.	ALA4384671	B	Inhibition of GST-fused MNK2a (unknown origin) expressed in HEK293 cells using eIF4E as substrate after 30 mins by Western blot analysis	Homo sapiens	2	ALA4382255	cell-based format	Scientific Literature
16.	ALA4384672	B	Inhibition of MNK in human MDA-MB-231 cells assessed as decrease in eIF4E phosphorylation at 1 uM after 2 hrs by Western blot analysis	Homo sapiens	2	ALA4382255	cell-based format	Scientific Literature
17.	ALA4384673	F	Antimigratory activity against human MDA-MB-231 cells after 24 hrs by DAPI staining-based Transwell assay	Homo sapiens	2	ALA4382255	cell-based format	Scientific Literature
18.	ALA4384676	A	Effect on total eIF4E protein level in mouse 3T3L1 cells at 3 uM after 2 hrs by Western blot analysis	Mus musculus	25	ALA4382255	cell-based format	Scientific Literature
19.	ALA4384679	B	Inhibition of MNK2 in MNK1-knockout C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 0.5 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	single protein format	Scientific Literature
20.	ALA4384680	B	Inhibition of MNK2 in MNK1-knockout C57BL/6J mouse embryonic fibroblasts assessed as decrease in eIF4E phosphorylation at 0.003 to 0.03 uM after 2 hrs by Western blot analysis	Mus musculus	2	ALA4382255	single protein format	Scientific Literature