#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888404	B	SGLT2 Inhibition Assay: For sodium-dependent glucose transport assay, cells expressing hSGLT2 were seeded into a 96-well culture plate at a density of 5×104 cells/well in RPMI medium 1640 containing 10% fetal bovine serum. The cells were used 1 day after plating. They were incubated in pretreatment buffer (10 mM HEPES, 5 mM Tris, 140 mM choline chloride, 2 mM KCl, 1 mM CaCl2, and 1 mM MgCl2, pH 7.4) at 37 °C. for 10 min. They were then incubated in uptake buffer (10 mM HEPES, 5 mM Tris, 140 mM NaCl, 2 mM KCl, 1 mM CaCl2, 1 mM MgCl2, and 1 mM 14C-nonlabeled AMG pH 7.4) containing 14C-labeled AMG (8 μM) and the inventive compound or dimethyl sulfoxide (DMSO) vehicle at 37 °C. for 2 h. Cells were washed twice with washing buffer (pretreatment buffer containing 10 mM AMG at room temperature) and then the radioactivity was measured using a liquid scintillation counter.	Homo sapiens	65	ALA3886697	assay format	BindingDB Database
2.	ALA3888405	B	SGLT1 Inhibition Assay: For sodium-dependent glucose transport assay, cells expressing hSGLT1 were seeded into a 96-well culture plate at a density of 5×104 cells/well in RPMI medium 1640 containing 10% fetal bovine serum. The cells were used 1 day after plating. They were incubated in pretreatment buffer (10 mM HEPES, 5 mM Tris, 140 mM choline chloride, 2 mM KCl, 1 mM CaCl2, and 1 mM MgCl2, pH 7.4) at 37 °C. for 10 min. They were then incubated in uptake buffer (10 mM HEPES, 5 mM Tris, 140 mM NaCl, 2 mM KCl, 1 mM CaCl2, 1 mM MgCl2, and 1 mM 14C-nonlabeled AMG pH 7.4) containing 14C-labeled (8 μM) and inhibitor or dimethyl sulfoxide (DMSO) vehicle at 37 °C. for 2 h. Cells were washed twice with washing buffer (pretreatment buffer containing 10 mM AMG at room temperature) and then the radioactivity was measured using a liquid scintillation counter.	Homo sapiens	65	ALA3886697	assay format	BindingDB Database
3.	ALA5144623	B	Inhibition of human SGLT2 expressed in CHO cells by methyl-alpha-D-glucopyranoside assay	Homo sapiens	20	ALA5143532	cell-based format	Scientific Literature
4.	ALA5144624	B	Inhibition of human SGLT1 expressed in CHO cells by methyl-alpha-D-glucopyranoside assay	Homo sapiens	14	ALA5143532	cell-based format	Scientific Literature
5.	ALA5144625	B	Selectivity index, ratio of IC50 for human SGLT1 expressed in CHO cells to IC50 for human SGLT2 expressed in CHO cells		14	ALA5143532	cell-based format	Scientific Literature
6.	ALA5144626	A	Metabolic stability in mouse by LC-MS/MS analysis	Mus musculus	9	ALA5143532	assay format	Scientific Literature