# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3742870 | B | Binding affinity to human GST-tagged TNF extracellular domain expressed in Escherichia coli BL21(DE3)pLysS cells by fluorescence spectrophotometric analysis | Homo sapiens | 41 | cell-based format | Scientific Literature | ||
2. | ALA3742872 | B | Inhibition of human TNF assessed as reduction in TNF-induced cell death of actinomycin D activated mouse L929 cells preincubated for 30 mins followed by cell addition measured after 18 to 24 hrs by crystal violet staining based spectrophotometric analysis | Homo sapiens | 42 | cell-based format | Scientific Literature | ||
3. | ALA3742873 | B | Binding affinity to recombinant human TNF assessed as inhibition of TNF-TNFR1 interaction incubated for 1 hr by ELISA | Homo sapiens | 42 | single protein format | Scientific Literature | ||
4. | ALA4671301 | B | Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in RANKL/M-CSF-induced TRAP activity at 5 uM preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days | Homo sapiens | 37 | cell-based format | Scientific Literature | ||
5. | ALA4671305 | A | Cytotoxicity against mouse BMMC assessed as reduction in cell viability measured after 48 hrs by MTT assay | Mus musculus | 46 | cell-based format | Scientific Literature | ||
6. | ALA4671307 | B | Binding affinity to GST-fused human RANKL extracellular domain (Lys159 to Asp317 residues) expressed in Escherichia coli BL21(DE3) pLysS by fluorescence spectrophotometric assay | Homo sapiens | 40 | assay format | Scientific Literature |