# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3744013 | Binding | Inhibition of recombinant human legumain using Z-Ala-Ala-Asn-MCA substrate measured every 2 mins for 60 mins by microplate reader analysis | Homo sapiens | 29 | ALA3739391 | single protein format | Scientific Literature | |
2. | ALA3744014 | Binding | Inhibition of legumain in human MCF7 cells using Z-Ala-Ala-Asn-MCA substrate at 30 uM incubated for 24 hrs by microplate reader analysis | Homo sapiens | 28 | ALA3739391 | cell-based format | Scientific Literature | |
3. | ALA3744015 | Functional | Cytotoxicity against human MCF7 cells assessed as cell death at 30 uM incubated for 96 hrs by MTT assay | Homo sapiens | 3 | ALA3739391 | cell-based format | Scientific Literature | |
4. | ALA3744016 | Functional | Cytotoxicity against human T47D cells assessed as cell viability at 30 uM incubated for 96 hrs by MTT assay | Homo sapiens | 1 | ALA3739391 | cell-based format | Scientific Literature | |
5. | ALA3744017 | Functional | Cytotoxicity against human PC3 cells assessed as cell viability at 30 uM incubated for 96 hrs by MTT assay | Homo sapiens | 1 | ALA3739391 | cell-based format | Scientific Literature | |
6. | ALA3744216 | Functional | Cytotoxicity against human DU145 cells assessed as cell viability at 30 uM incubated for 96 hrs by MTT assay | Homo sapiens | 1 | ALA3739391 | cell-based format | Scientific Literature | |
7. | ALA3744217 | Functional | Antimetastatic activity in human MCF7 cells assessed as inhibition of colony formation efficiency at 5 uM after 2 weeks by soft agar anchorage-independent growth assay | Homo sapiens | 1 | ALA3739391 | cell-based format | Scientific Literature | |
8. | ALA3744218 | Functional | Antimetastatic activity in human PC3 cells assessed as inhibition of colony formation efficiency at 2.5 uM after 2 weeks by soft agar anchorage-independent growth assay | Homo sapiens | 1 | ALA3739391 | cell-based format | Scientific Literature | |
9. | ALA3744219 | Binding | Inhibition of recombinant caspase 8 (unknown origin) using IETD-AFC fluorogenic substrate | Homo sapiens | 3 | ALA3739391 | single protein format | Scientific Literature | |
10. | ALA3744220 | Binding | Inhibition of recombinant cathepsin S (unknown origin) using ZVVR-AMC fluorogenic substrate | Homo sapiens | 3 | ALA3739391 | single protein format | Scientific Literature | |
11. | ALA3744221 | Binding | Inhibition of recombinant cathepsin B (unknown origin) using Z-Leu-Arg-AMC fluorogenic substrate | Homo sapiens | 3 | ALA3739391 | single protein format | Scientific Literature | |
12. | ALA3744222 | Binding | Inhibition of recombinant caspase 3 (unknown origin) using DEVD-AFC fluorogenic substrate | Homo sapiens | 3 | ALA3739391 | single protein format | Scientific Literature | |
13. | ALA3744223 | Binding | Inhibition of USP17 in human HEK293T cells using fluorogenic substrate ub-rhodamine110 preincubated for 30 mins followed by substrate addition measured every 4 mins for 1 hr by microplate reader analysis | Homo sapiens | 3 | ALA3739391 | cell-based format | Scientific Literature | |
14. | ALA3744224 | Functional | Cytotoxicity against human MCF10A cells assessed as cell death at 150 uM incubated for 96 hrs by MTT assay | Homo sapiens | 3 | ALA3739391 | cell-based format | Scientific Literature | |
15. | ALA3744225 | Binding | Inhibition of legumain in human MCF7 cells using Z-Ala-Ala-Asn-MCA substrate at 30 uM incubated for 48 hrs by microplate reader analysis | Homo sapiens | 1 | ALA3739391 | cell-based format | Scientific Literature | |
16. | ALA4341722 | ADME | Intrinsic clearance in mouse liver microsomes at 1 uM in presence of NADPH incubated for 15 mins by LC/MS/MS method | Mus musculus | 12 | ALA4340539 | microsome format | Scientific Literature | |
17. | ALA4341723 | Physicochemical | Kinetic solubility of the compound at pH 7.4 PBS up to 200 uM | 12 | ALA4340539 | small-molecule physicochemical format | Scientific Literature |