# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3756828 | A | Cytotoxicity against mock-infected human Huh7.5 cells assessed as reduction in cell viability incubated for 5 hrs by MTT assay | Homo sapiens | 22 | ALA3751796 | cell-based format | Scientific Literature | |
2. | ALA3756829 | F | Antiviral activity against recombinant HCV genotype 2a JFH1 infected in human Huh7 cells incubated for 5 hrs measured on day 3 post infection by luciferase reporter gene assay | Hepacivirus hominis | 22 | ALA3751796 | organism-based format | Scientific Literature | |
3. | ALA3756830 | U | Selectivity index, ratio of CC50 for mock-infected human Huh7.5 cells to EC50 for recombinant HCV genotype 2a JFH1 | 16 | ALA3751796 | cell-based format | Scientific Literature | ||
4. | ALA3756831 | B | Binding affinity to Cyclophilin A (unknown origin) at 0.625 nM to 10 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA3751796 | single protein format | Scientific Literature | |
5. | ALA3756832 | B | Binding affinity to Cyclophilin B (unknown origin) at 10 to 30 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA3751796 | single protein format | Scientific Literature | |
6. | ALA3756833 | B | Binding affinity to Cyclophilin A (unknown origin) by surface plasmon resonance analysis | Homo sapiens | 2 | ALA3751796 | single protein format | Scientific Literature | |
7. | ALA3756834 | B | Binding affinity to Cyclophilin B (unknown origin) by surface plasmon resonance analysis | Homo sapiens | 2 | ALA3751796 | single protein format | Scientific Literature | |
8. | ALA3756835 | A | Cytotoxicity against BALB/c mouse splenocytes assessed as reduction in cell viability at 1 to 10 uM | Mus musculus | 2 | ALA3751796 | cell-based format | Scientific Literature | |
9. | ALA3756836 | B | Inhibition of calcium-dependent calcineurin phosphatase activity in ionomycin-stimulated BALB/c mouse splenocytes assessed as reduction in dephosphorylation of R2 phosphopeptide at 1 to 10 uM preincubated for 20 hrs followed by ionomycin stimulation measured after 4 hrs | Mus musculus | 2 | ALA3751796 | assay format | Scientific Literature | |
10. | ALA3756837 | B | Activation of calcium-dependent calcineurin phosphatase activity in ionomycin-stimulated BALB/c mouse splenocytes assessed as dephosphorylation of NFAT1 at 1 to 10 uM preincubated for 20 hrs followed by ionomycin stimulation measured after 4 hrs by Western blot analysis | Mus musculus | 1 | ALA3751796 | assay format | Scientific Literature | |
11. | ALA3756838 | B | Activation of calcium-dependent calcineurin phosphatase activity in ionomycin-stimulated BALB/c mouse splenocytes assessed as nuclear translocation of NFAT1 at 5 uM preincubated for 20 hrs followed by ionomycin stimulation measured after 4 hrs by DAPI staining based confocal microscopy | Mus musculus | 1 | ALA3751796 | assay format | Scientific Literature | |
12. | ALA3756839 | B | Activation of calcium-dependent calcineurin phosphatase activity in ionomycin-stimulated BALB/c mouse assessed as production of IL-2 at 1 to 10 uM after 24 hrs by ELISA | Mus musculus | 2 | ALA3751796 | assay format | Scientific Literature | |
13. | ALA3756840 | B | Inhibition of Cyclophilin A in human Huh7.5 cells infected with HCV assessed as reduction in viral RNA replication level at 0.5 to 4 uM by luciferase reporter gene assay | Homo sapiens | 1 | ALA3751796 | cell-based format | Scientific Literature | |
14. | ALA3756841 | F | Antiviral activity against HCV genotype 1b Con1 infected in human Huh7.5 cells assessed as inhibition of NS5A expression in total cell lysate at 2 uM by Western blot analysis | Hepacivirus hominis | 1 | ALA3751796 | organism-based format | Scientific Literature | |
15. | ALA3756842 | F | Antiviral activity against HCV genotype 1b Con1 infected in human Huh7.5 cells assessed as inhibition of NS5B expression in total cell lysate at 2 uM by Western blot analysis | Hepacivirus hominis | 1 | ALA3751796 | organism-based format | Scientific Literature | |
16. | ALA3756843 | F | Antiviral activity against HCV genotype 1b Con1 infected in human Huh7.5 cells assessed as inhibition of NS5A expression at 2 uM by DAPI staining based confocal microscopy | Hepacivirus hominis | 1 | ALA3751796 | organism-based format | Scientific Literature | |
17. | ALA3756844 | F | Antiviral activity against HCV genotype 1b Con1 infected in human Huh7.5 cells assessed as inhibition of NS5B expression at 2 uM by DAPI staining based confocal microscopy | Hepacivirus hominis | 1 | ALA3751796 | organism-based format | Scientific Literature | |
18. | ALA3757000 | F | Antiviral activity against HCV genotype 1b Con1 infected in human Huh7.5 cells expressing GFP-fused NS5A measured 72 hrs post infection by hoechst 33342 staining based confocal microscopy | Hepacivirus hominis | 1 | ALA3751796 | organism-based format | Scientific Literature | |
19. | ALA3757001 | F | Antiviral activity against HCV genotype 2a JFH1 infected in human Huh7.5 cells expressing GFP-fused NS5A measured 72 hrs post infection by hoechst 33342 staining based confocal microscopy | Hepacivirus hominis | 1 | ALA3751796 | organism-based format | Scientific Literature | |
20. | ALA3757002 | A | Cytotoxicity against HCV genotype 1b Con1 infected human Huh7.5 cells expressing GFP-fused NS5A assessed as reduction in cell viability measured 72 hrs post infection by hoechst 33342 staining based confocal microscopy | Homo sapiens | 1 | ALA3751796 | cell-based format | Scientific Literature |