# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3776538 | B | Inhibition of full length N-terminal His-tagged human recombinant LSD1 (1 to 852 residues) expressed in Escherichia coli BL21(DE3) cells using H3K4me2 peptide as substrate preincubated for 5 mins followed by substrate addition measured for 30 mins by peroxidase coupled assay | Homo sapiens | 10 | ALA3774333 | cell-based format | Scientific Literature | |
2. | ALA3777876 | B | Inhibition of LSD1 in human HeLa cells assessed as H3K4me2/H3 ratio at 20 uM after 24 hrs by Western blot analysis in absence of fetal bovine serum (Rvb = 1 No_unit) | Homo sapiens | 6 | ALA3774333 | cell-based format | Scientific Literature | |
3. | ALA3778076 | F | Antiproliferative activity against human HeLa cells assessed as reduction in cell viability at 300 uM treated for 24 hrs measured after 48 hrs by alamar blue assay in absence of fetal bovine serum relative to control | Homo sapiens | 1 | ALA3774333 | cell-based format | Scientific Literature | |
4. | ALA3778078 | F | Antiproliferative activity against human HeLa cells assessed as reduction in cell viability at 100 uM treated for 24 hrs measured after 48 hrs by alamar blue assay in absence of fetal bovine serum | Homo sapiens | 1 | ALA3774333 | cell-based format | Scientific Literature | |
5. | ALA4220803 | F | Cytotoxicity against mouse F9 cells at 5 uM after 16 to 30 hrs hrs by microscopic analysis | Mus musculus | 1 | ALA4219071 | cell-based format | Scientific Literature | |
6. | ALA4220804 | F | Cytotoxicity against human HCT116 cells at 5 uM after 16 to 30 hrs hrs by microscopic analysis | Homo sapiens | 1 | ALA4219071 | cell-based format | Scientific Literature | |
7. | ALA4220805 | F | Cytotoxicity against human PA1 cells at 5 uM after 16 to 30 hrs hrs by microscopic analysis | Homo sapiens | 1 | ALA4219071 | cell-based format | Scientific Literature | |
8. | ALA4220806 | A | Cytotoxicity against mouse NIH/3T3 cells at 5 uM after 16 to 30 hrs hrs by microscopic analysis | Mus musculus | 1 | ALA4219071 | cell-based format | Scientific Literature | |
9. | ALA4253684 | B | Inhibition of LSD1 (unknown origin) | Homo sapiens | 14 | ALA4251603 | single protein format | Scientific Literature | |
10. | ALA4253691 | F | Induction of cell death in human Rh30 cells at 50 to 100 nM after 72 hrs by propidium iodide/Hoechst 33342 staining based fluorescence microscopic analysis | Homo sapiens | 3 | ALA4251603 | cell-based format | Scientific Literature | |
11. | ALA4253692 | F | Potentiation of JNJ-26481585-induced cell death in human Rh30 cells after 72 hrs by propidium iodide/Hoechst 33342 staining based fluorescence microscopic analysis | Homo sapiens | 3 | ALA4251603 | cell-based format | Scientific Literature | |
12. | ALA4253693 | B | Inhibition of LSD1 in human THP1 cells assessed as increase in CD84+ level at 0.5 uM after 72 hrs by APC/7AAD staining based flow cytometry | Homo sapiens | 7 | ALA4251603 | cell-based format | Scientific Literature |