# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3783455 | B | Inhibition of FLAP in Ca+2 ionophore A23187 stimulated human neutrophils assessed as remaining 5-LO activity preincubated for 15 mins at 10 uM measured after 10 mins by RP-HPLC analysis | Homo sapiens | 16 | ALA3779871 | single protein format | Scientific Literature | |
2. | ALA3783530 | B | Inhibition of FLAP in Ca+2 ionophore A23187 stimulated human neutrophils assessed as 5-LO product formation preincubated for 15 mins measured after 10 mins by RP-HPLC analysis | Homo sapiens | 16 | ALA3779871 | single protein format | Scientific Literature | |
3. | ALA3783531 | B | Inhibition of recombinant human 5-LO expressed in Escherichia coli BL21 assessed as 5-LO product formation preincubated for 15 mins followed by addition of arachidonic acid as substrate measured after 10 mins by RP-HPLC analysis | Homo sapiens | 16 | ALA3779871 | assay format | Scientific Literature | |
4. | ALA3783532 | A | Acute cytotoxicity against human neutrophils assessed as cell viability measured for 30 mins by trypan blue exclusion method | Homo sapiens | 16 | ALA3779871 | cell-based format | Scientific Literature | |
5. | ALA4152533 | B | Inhibition of 5-LOX (unknown origin) | Homo sapiens | 4 | ALA4152186 | single protein format | Scientific Literature | |
6. | ALA5244394 | B | Inhibition of FLAP in human PMNL cells assessed as reduction in all-trans isomers of LTB4 and 5-HETE formation preincubated with enzyme for 15 mins followed by Ca2+ ionophore-A23187 and measured after 10 mins by HPLC analysis | Homo sapiens | 1 | ALA5244204 | cell-based format | Scientific Literature | |
7. | ALA5244395 | B | Inhibition of FLAP in human monocyte assessed as reduction in all-trans isomers of LTB4 and 5-HETE formation preincubated with enzyme for 15 mins followed by Ca2+ ionophore-A23187 and measured after 10 mins by HPLC analysis | Homo sapiens | 1 | ALA5244204 | cell-based format | Scientific Literature |