# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3791442 | Binding | Inhibition of human KDM4A (1 to 359 residues) expressed in competent Escherichia coli BL21-CodonPlus-RIL cells using H3K9me3 as substrate preincubated for 10 mins followed by substrate addition measured after 1 hr by FDH coupled enzyme assay | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
2. | ALA3791443 | Binding | Inhibition of human KDM4A (1 to 359 residues) expressed in competent Escherichia coli BL21-CodonPlus-RIL cells using H3K9me3 as substrate preincubated for 10 mins followed by substrate addition measured after 45 mins by TR-FRET assay | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
3. | ALA3791444 | Binding | Inhibition of full length human C-terminal FLAG-tagged KDM5A (1 to 1090 residues) expressed in baculovirus infected sf9 cells using H3K4me3 (1 to 21 residues) substrate preincubated for 10 mins followed by substrate addition measured after 45 mins by TR-FRET assay | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
4. | ALA3791445 | Binding | Inhibition of human C-terminal FLAG-tagged KDM6B (1043 residues) expressed in baculovirus infected sf9 cells using H3K27me3 (21 to 44 residues) substrate preincubated for 10 mins followed by substrate addition measured after 45 mins by TR-FRET assay | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
5. | ALA3791456 | Binding | Selectivity ratio of IC50 for full length human C-terminal FLAG-tagged KDM5A (1 to 1090 residues) expressed in baculovirus infected sf9 cells to IC50 for human KDM4A (1 to 359 residues) expressed in competent Escherichia coli BL21-CodonPlus-RIL cells | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
6. | ALA3791457 | Binding | Selectivity ratio of IC50 for human C-terminal FLAG-tagged KDM6B (1043 residues) expressed in baculovirus infected sf9 cells to IC50 for human KDM4A (1 to 359 residues) expressed in competent Escherichia coli BL21-CodonPlus-RIL cells | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
7. | ALA3791459 | Binding | Competitive inhibition of human KDM4A (1 to 359 residues) expressed in competent Escherichia coli BL21-CodonPlus-RIL cells using 2-oxoglutarate as substrate preincubated for 10 mins followed by substrate addition measured after 45 mins by TR-FRET assay | Homo sapiens | 1 | ALA3784917 | cell-based format | Scientific Literature | |
8. | ALA5141136 | Binding | Inhibition of KDM4A (unknown origin) measured by formaldehyde dehydrogenase (FDH)-coupled assay | Homo sapiens | 1 | ALA5137065 | single protein format | Scientific Literature | |
9. | ALA5141137 | Binding | Inhibition of KDM4A (unknown origin) measured by antibody-based LANCE Ultra assay | Homo sapiens | 1 | ALA5137065 | single protein format | Scientific Literature |