# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3801076 | F | Antiproliferative activity against human p53 +/+ HCT116 cells measured after 72 hrs by MTS assay | Homo sapiens | 32 | cell-based format | Scientific Literature | ||
2. | ALA3801077 | F | Antiproliferative activity against human p53 -/- HCT116 cells measured after 72 hrs by MTS assay | Homo sapiens | 18 | cell-based format | Scientific Literature | ||
3. | ALA3801078 | F | Antiproliferative activity against human HepG2 cells expressing wild-type P53 measured after 72 hrs by MTS assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
4. | ALA3801079 | F | Antiproliferative activity against human SW620 cells expressing P53 mutant measured after 72 hrs by MTS assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
5. | ALA3801080 | B | Inhibition of p53-MDM2 interaction (unknown origin) in human p53 -/- HCT116 cells co-transfected with V1-p53/MDM2-V2 plasmid assessed as interaction-induced fluorescence level at 5 uM by Venus-based bimolecular fluorescence complementation assay (Rvb = 34.18 %) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
6. | ALA3801081 | B | Inhibition of p53-MDM2 interaction (unknown origin) in human p53 -/- HCT116 cells co-transfected with V1-p53/MDM2-V2 plasmid interaction-induced fluorescence level at 10 uM by Venus-based bimolecular fluorescence complementation assay (Rvb = 34.18 %) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
7. | ALA3801279 | B | Inhibition of p53-MDM2 interaction (unknown origin) in human p53 -/- HCT116 cells co-transfected with V1-p53/MDM2-V2 plasmid interaction-induced fluorescence level at 20 uM by Venus-based bimolecular fluorescence complementation assay (Rvb = 34.18 %) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
8. | ALA3801280 | B | Induction of p53 stabilization in human p53 +/+ HCT116 cells assessed as increase in p53 level at 5 uM after 24 hrs by immunoblot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
9. | ALA3801281 | B | Induction of p53 stabilization in human p53 +/+ HCT116 cells assessed as increase in MDM2 level at 5 uM after 24 hrs by immunoblot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA3801282 | B | Induction of p53 transcriptional activation in human p53 +/+ HCT116 cells assessed as PUMA transactivation at 10 uM after 24 hrs by dual-luciferase reporter assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
11. | ALA3801283 | B | Induction of p53 transcriptional activation in human p53 +/+ HCT116 cells assessed as p21/WAF1 transactivation at 10 uM after 24 hrs by dual-luciferase reporter assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
12. | ALA3801284 | F | Induction of apoptosis in human p53 +/+ HCT116 cells assessed as activation of caspase3 at 5 or 10 uM after 24 hrs by Caspase-Glo 3/7 assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
13. | ALA3801285 | F | Induction of apoptosis in human p53 +/+ HCT116 cells assessed as activation of caspase7 at 5 or 10 uM after 24 hrs by Caspase-Glo 3/7 assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
14. | ALA3801286 | F | Induction of apoptosis in human p53 +/+ HCT116 cells assessed as increase in PARP cleavage at 5 or 10 uM after 24 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
15. | ALA3801287 | B | Induction of p53 phosphorylation at serine-15 in human p53 +/+ HCT 116 cells bat 5 or 10 uM after 24 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
16. | ALA3801288 | P | Chemical stability in phosphate buffer at pH 7.4 and 37 degC measured for 3 days by HPLC analysis | 1 | small-molecule physicochemical format | Scientific Literature | |||
17. | ALA3801289 | A | Metabolic stability at human plasma assessed as compound degradation at 37 degC after 72 hrs by HPLC analysis | Homo sapiens | 1 | cell-free format | Scientific Literature | ||
18. | ALA3801290 | A | Half life in Sprague-Dawley rat liver microsomes at 37 degC in presence of NADPH generating system by HPLC analysis | Rattus norvegicus | 1 | tissue-based format | Scientific Literature |