# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3801317 | B | Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli assessed as L-[3H]-citrulline formation at 1 mM using L-[3H]-arginine as substrate after 30 mins by liquid scintillation spectroscopy | Homo sapiens | 31 | ALA3797128 | assay format | Scientific Literature | |
2. | ALA3801318 | B | Inhibition of recombinant nNOS (unknown origin) assessed as L-[3H]-citrulline formation at 1 mM using L-[3H]-arginine as substrate after 30 mins by liquid scintillation spectroscopy | Homo sapiens | 19 | ALA3797128 | single protein format | Scientific Literature | |
3. | ALA3801319 | B | Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli assessed as L-[3H]-citrulline formation using L-[3H]-arginine as substrate after 30 mins by liquid scintillation spectroscopy | Homo sapiens | 6 | ALA3797128 | assay format | Scientific Literature | |
4. | ALA3801320 | B | Inhibition of recombinant nNOS (unknown origin) assessed as L-[3H]-citrulline formation using L-[3H]-arginine as substrate after 30 mins by liquid scintillation spectroscopy | Homo sapiens | 6 | ALA3797128 | single protein format | Scientific Literature | |
5. | ALA3801321 | B | Inhibition of eNOS in HUVEC cells assessed as A23187 stimulated nitric oxide production at 100 umol/L preincubated for 30 mins using L-arginine as substrate for 5 mins followed A23187 addition measured after 30 mins by DAF2 dye based spectrofluorimetry | Homo sapiens | 1 | ALA3797128 | cell-based format | Scientific Literature | |
6. | ALA3801322 | B | Inhibition of eNOS in Wistar rat aortic rings assessed as effect on acetylcholine evoked endothelial dependent relaxation of precontracted aortic rings at 100 umol/l after 30 mins | Rattus norvegicus | 1 | ALA3797128 | single protein format | Scientific Literature | |
7. | ALA3801324 | A | Cytotoxicity against HUVEC cells assessed as reduction in cell viability at >1 mM after 30 mins by MTT assay | Homo sapiens | 1 | ALA3797128 | cell-based format | Scientific Literature | |
8. | ALA4809865 | B | Inhibition of N-terminal His-tagged human recombinant iNOS expressed in Escherichia coli assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate at 1 mM incubated for 30 mins by liquid scintillation method relative to control | Homo sapiens | 19 | ALA4808198 | assay format | Scientific Literature | |
9. | ALA4809866 | B | Inhibition of human recombinant nNOS expressed in Escherichia coli assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate at 1 mM incubated for 30 mins by liquid scintillation method relative to control | Homo sapiens | 19 | ALA4808198 | assay format | Scientific Literature | |
10. | ALA4809867 | B | Inhibition of N-terminal His-tagged human recombinant iNOS expressed in Escherichia coli assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate incubated for 30 min by ELISA | Homo sapiens | 8 | ALA4808198 | assay format | Scientific Literature | |
11. | ALA4809868 | B | Inhibition of human recombinant nNOS expressed in Escherichia coli assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate incubated for 30 min by ELISA | Homo sapiens | 8 | ALA4808198 | assay format | Scientific Literature |