| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3801197 | B | Displacement of FAM-Bid peptide from N-terminal 8x His-tagged MCl-1 (unknown origin) expressed in Escherichia coli BL21 (DE3) after 30 mins by fluorescence polarization assay | Homo sapiens | 22 | assay format | Scientific Literature | ||
| 2. | ALA3801198 | B | Displacement of FAM-Bid peptide from N-terminal 8x His-tagged Bcl-2 (unknown origin) expressed in Escherichia coli BL21 (DE3) after 30 mins by fluorescence polarization assay | Homo sapiens | 22 | assay format | Scientific Literature | ||
| 3. | ALA3801199 | B | Displacement of FAM-p53TAD peptide from N-terminal 8x His-tagged human MDM2 (25 to 108 residues) expressed in Escherichia coli BL21 (DE3) after 30 mins by fluorescence polarization assay | Homo sapiens | 22 | assay format | Scientific Literature | ||
| 4. | ALA3801200 | B | Binding affinity to N-terminal 8x His-tagged human MDM2 (25 to 108 residues) expressed in Escherichia coli BL21 (DE3) by isothermal titration microcalorimetric method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA3801201 | B | Binding affinity to N-terminal 8x His-tagged Bcl-2 (unknown origin) expressed in Escherichia coli BL21 (DE3) by isothermal titration microcalorimetric method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA3801202 | B | Binding affinity to N-terminal 8x His-tagged MCl-1 (unknown origin) expressed in Escherichia coli BL21 (DE3) by isothermal titration microcalorimetric method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 7. | ALA3801203 | B | Binding affinity to 15N-labeled human MCl-1 (172 to 321 residues) BimBH3 binding domain expressed in Escherichia coli BL21 at 1:1 compound to protein ratio at 35 degC by 1H-15N HSQC NMR spectroscopic method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA3801204 | B | Binding affinity to 15N-labeled human MDM2 (1 to 118 residues) p53TAD binding domain expressed in Escherichia coli BL21 at 1:1 compound to protein ratio at 15 degC by 1H-15N HSQC NMR spectroscopic method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA3801205 | B | Inhibition of MDM2 in human HCT116 p53+/+ cells assessed as growth inhibition | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA3801206 | B | Inhibition of Bcl2 in human DMS53 cells assessed as growth inhibition | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA3801207 | B | Inhibition of MDM2-p53 interaction in human HCT116 p53+/+ cells assessed as upregulation of p53 expression after 12 hrs by immunoblotting method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA3801208 | F | Cell cycle arrest in human HCT116 p53+/+ cells assessed as accumulation at | Homo sapiens | 3 | cell-based format | Scientific Literature | | ||
| 13. | ALA3801209 | B | Inhibition of MDM2-p53 interaction in human HCT116 p53+/+ cells assessed as upregulation of MDM2 expression after 12 hrs by immunoblotting method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 14. | ALA3801210 | B | Inhibition of MDM2-p53 interaction in human HCT116 p53+/+ cells assessed as upregulation of p21 expression after 12 hrs by immunoblotting method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 15. | ALA3801405 | F | Effect on MDM2 expression in human HCT116 p53-/- cells at 10 to 20 uM after 12 hrs by immunoblotting method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA3801406 | F | Effect on p53 expression in human HCT116 p53-/- cells at 10 to 20 uM after 12 hrs by immunoblotting method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA3801407 | F | Effect on p21 expression in human HCT116 p53-/- cells at 10 to 20 uM after 12 hrs by immunoblotting method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA3801408 | B | Induction of p53 phosphorylation at Ser15 in human HCT116 p53+/+ cells at 5 uM after 24 hrs by immunoblotting method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA3801409 | F | Upregulation of p53 mRNA expression in human HCT116 p53+/+ cells at 10 uM after 12 hrs by qPCR method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA3801410 | B | Activation of p53 in human HCT116 p53+/+ cells assessed as upregulation of p21 mRNA expression at 10 uM after 12 hrs by qPCR method relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature |
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