Inhibition of DAAO in porcine kidney homogenate using D-alanine as substrate assessed as pyruvate production incubated for 5 mins by microplate reader analysis
Analgesic activity in Wistar rat assessed as inhibition of formalin-induced paw flinch response in acute phase up to 3 ug, it administered as bolus injection 30 mins prior to formalin challenge measured within 10 mins relative to normal saline control
Analgesic activity in it dosed Wistar rat assessed as inhibition of formalin-induced paw flinch response in tonic phase administered as bolus injection 30 mins prior to formalin challenge measured from 10 to 90 mins relative to normal saline control
Analgesic activity in Wistar rat assessed as inhibition of NMDA-induced pain at 10 ug, it administered 30 mins prior to NMDA challenge measured for 10 mins
Analgesic activity in Wistar rat spinal nerve ligation-induced neuropathic pain model assessed as reduction in mechanical allodynia assessed as increase in ipsilateral paw withdrawal response at 30 ug, it measured after 1 hr
Analgesic activity in Wistar rat spinal nerve ligation-induced neuropathic pain model assessed as reduction in heat hyperalgesia assessed as increase in ipsilateral paw withdrawal response at 30 ug, it measured after 1 hr
Analgesic activity in Wistar rat spinal nerve ligation-induced neuropathic pain model assessed as reduction in mechanical allodynia assessed as increase in ipsilateral paw withdrawal response at 30 ug, it measured after 0.5 to 4 hrs
Analgesic activity in Wistar rat spinal nerve ligation-induced neuropathic pain model assessed as reduction in heat hyperalgesia assessed as increase in ipsilateral paw withdrawal response at 30 ug, it measured after 0.5 to 4 hrs
Analgesic activity in Swiss mouse assessed as inhibition of formalin-induced tonic pain at 10 mg/kg administered as oral gavage 30 mins prior to formalin challenge measured from 20 to 40 mins relative to normal saline control
Analgesic activity in Swiss mouse assessed as inhibition of formalin-induced acute pain at 0.3 to 10 mg/kg, sc administered 30 mins prior to formalin challenge measured within 5 mins relative to normal saline control
Analgesic activity in sc dosed Swiss mouse assessed as inhibition of formalin-induced tonic pain administered 30 mins prior to formalin challenge measured from 20 to 40 mins relative to normal saline control
Attenuation of morphine analgesic tolerance in Swiss mouse assessed as latency of licking/biting/jumping at 10 ug, it bid coadministered with morphine at 12 hr intervals for 7 days by hot-plate test
Reversal of morphine analgesic tolerance in 10 ug, it BID for 7 days morphine pre-treated Swiss mouse assessed as latency of licking/biting/jumping at 10 ug, it administered as single bolus injection 15 mins prior to morphine challenge on day 8 by hot-plate test