# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3877061 | B | Binding affinity to recombinant human C-terminal His6-tagged Bcl2A1 (1 to 152 residues) expressed in Escherichia coli assessed as adduct formation at 0.5 to 10 uM measured after 2 hrs by denaturing SDS-PAGE analysis | Homo sapiens | 1 | ALA3875227 | assay format | Scientific Literature | |
2. | ALA3877063 | B | Binding affinity to recombinant human C-terminal His6-tagged Bcl2A1 (1 to 152 residues) at Cys55 residue expressed in Escherichia coli assessed as adduct formation at 8 uM incubated 2 hrs prior to SDS-PAGE in-gel trypsin digestion by LC-MS/MS analysis | Homo sapiens | 1 | ALA3875227 | assay format | Scientific Literature | |
3. | ALA3877064 | B | Binding affinity to Mcl1 BH3 binding site (unknown origin) at 10 uM measured after 5 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA3875227 | single protein format | Scientific Literature | |
4. | ALA3877065 | B | Binding affinity to Bcl1 BH3 binding site (unknown origin) at 10 uM measured after 5 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA3875227 | single protein format | Scientific Literature | |
5. | ALA3877066 | B | Binding affinity to Bcl-XL BH3 binding site (unknown origin) at 10 uM measured after 5 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA3875227 | single protein format | Scientific Literature | |
6. | ALA3877067 | P | Stability of the compound in DTT buffer at 50 uM and pH 7.2 measured after 1 to 17 hrs by mass spectra analysis | 1 | ALA3875227 | small-molecule physicochemical format | Scientific Literature | ||
7. | ALA3877068 | A | Metabolic stability in human serum at 25 uM measured up to 6 hrs | Homo sapiens | 2 | ALA3875227 | assay format | Scientific Literature | |
8. | ALA3877069 | B | Inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli assessed as half life of Bcl2A1 to FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding at 500 nM by fluorescence polarization assay | Homo sapiens | 1 | ALA3875227 | assay format | Scientific Literature | |
9. | ALA3877070 | B | Inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli at 500 nM measured after 90 mins by fluorescence polarization assay | Homo sapiens | 1 | ALA3875227 | assay format | Scientific Literature | |
10. | ALA3877071 | B | Inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 2 | ALA3875227 | assay format | Scientific Literature | |
11. | ALA3877073 | B | Irreversible inhibition of FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 binding to recombinant human Bcl2A1 (1 to 152 residues) BH3 binding site expressed in Escherichia coli preincubated for 1 hr with 2 fold diluted Bcl2A1 prior to FITC-betaA-DIIRNIARHLAQVGDSMRSI-NH2 addition measured every minute over 3.5 hrs by fluorescence polarization assay | Homo sapiens | 1 | ALA3875227 | assay format | Scientific Literature | |
12. | ALA3877077 | B | Binding affinity to Bcl2A1 in human U937 cells at 0.1 to 50 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA3875227 | cell-based format | Scientific Literature | |
13. | ALA3877078 | B | Binding affinity to Bcl2A1 in human U937 cells assessed as Bcl2A1 modification at 5 to 50 uM measured after overnight incubation by Western blot analysis | Homo sapiens | 2 | ALA3875227 | cell-based format | Scientific Literature | |
14. | ALA3877079 | B | Binding affinity to Bcl2A1 (unknown origin) overexpressed in human HeLa cells at 5 to 50 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA3875227 | cell-based format | Scientific Literature |