#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3887138	B	MGAT SPA Assay: MGAT2 enzyme was assayed using membranes isolated from Sf9 cells expressing the recombinant human MGAT2 cDNA with 2-monooleoylglycerol and [3H]-oleoyl-CoA as substrates as described by Seethala et al. [Anal. Biochem., 383(2):144-150 (Dec. 15, 2008)]. Briefly, the assays were conducted in 384-well plates in a total volume of 30 uL at 25° C. In each assay, 200 ng of recombinant human MGAT2 membrane was incubated with 10 uM of 2-monooleoylglycerol and 15 uM of [3H]-oleoyl-CoA in 100 mM potassium phosphate (pH 7.4) for 20 min with various concentrations of compounds delivered in DMSO. The assay was terminated by the addition of 20 ul of Stopping Solution (7.5 mg/ml Yttrium Oxide Polylysine beads, 3.3 mg/ml Fraction V BSA and 200 uM Mercuric chloride in 50 mM HEPES, pH 7.4). The signal was measured 1 h after quenching the reaction using LEADSEEKERSM for 5 minutes. To calculate the degree of inhibition, the zero level of enzyme activity (blank) was defined.		127	ALA3886182	tissue-based format	BindingDB Database
2.	ALA3887848	B	MGAT LCMS Assay: The MGAT enzyme reactions were performed in CORNING Falcon 96-well Polypropylene plates, in a total volume of 60 uL of 50 mM Potassium Phosphate buffer pH 7.4, containing a final concentration of 100 uM 2-oleoylglycerol, 15 uM oleoyl-Coenzyme A and 0.0013 ug/uL Human or Mouse MGAT-2 or 0.0026 ug/uL Rat recombinant MGAT-2 membranes expressed in Sf9 cells. Assay plates were run through a fully automated robotics system and shaken for 5 seconds every minute for a total 10 minutes. The reactions were then quenched with 120 uL of ice cold methanol containing 1 ug/mL 1,2-distearoyl-rac-glycerol as the internal standard. Plates were shaken for 2 minutes and spun down to remove protein precipitation. After the spin, samples were transferred to LC/MS compatible PCR plates. For LC/MS analysis, a ThermoFisher Surveyor pump, utilizing a Waters Symmetry C8, 50x2.1 mm column, was used for the chromatography of enzyme products.		262	ALA3886182	cell-based format	BindingDB Database
3.	ALA4845048	B	Inhibition of human recombinant MGAT2 expressed in Sf9 cell membrane using 2-monooleglycerol and [H3]-oleoyl-CoA as substrates incubated for 20 mins by scintillation proximity assay	Homo sapiens	59	ALA4842384	cell membrane format	Scientific Literature
4.	ALA4845049	B	Inhibition of mouse recombinant MGAT2 expressed in Sf9 cell membrane using 2-oleoylglycerol and oleoyl-CoA as substrates incubated for 10 mins by LCMS assay	Mus musculus	59	ALA4842384	cell membrane format	Scientific Literature
5.	ALA4845050	A	Metabolic stability in human liver microsomes assessed as compound remaining at 0.5 uM incubated for 10 mins	Homo sapiens	49	ALA4842384	microsome format	Scientific Literature
6.	ALA4845051	A	Metabolic stability in mouse liver microsomes assessed as compound remaining at 0.5 uM incubated for 10 mins	Mus musculus	49	ALA4842384	microsome format	Scientific Literature
7.	ALA4845052	B	Inhibition of rat recombinant MGAT2 expressed in Sf9 cell membrane using 2-oleoylglycerol and oleoyl-CoA as substrates incubated for 10 mins by LCMS assay	Rattus norvegicus	3	ALA4842384	cell membrane format	Scientific Literature
8.	ALA4845053	A	Metabolic stability in rat liver microsomes assessed as compound remaining at 0.5 uM incubated for 10 mins	Rattus norvegicus	3	ALA4842384	microsome format	Scientific Literature
9.	ALA4845054	A	Plasma protein binding in human assessed as unbound fraction	Homo sapiens	3	ALA4842384	cell-free format	Scientific Literature
10.	ALA4845055	A	Plasma protein binding in rat assessed as unbound fraction	Rattus norvegicus	3	ALA4842384	cell-free format	Scientific Literature
11.	ALA4845056	A	Plasma protein binding in mouse assessed as unbound fraction	Mus musculus	3	ALA4842384	cell-free format	Scientific Literature
12.	ALA4845057	P	Aqueous solubility of compound at pH 1		3	ALA4842384	small-molecule physicochemical format	Scientific Literature
13.	ALA4845058	P	Aqueous solubility of compound at pH 6.5		3	ALA4842384	small-molecule physicochemical format	Scientific Literature
14.	ALA4845059	P	Aqueous solubility of compound at pH 7.4		3	ALA4842384	small-molecule physicochemical format	Scientific Literature
15.	ALA4845060	A	Permeability of compound at pH 5.5 by PAMPA assay		3	ALA4842384	assay format	Scientific Literature
16.	ALA4845061	A	Permeability of compound at pH 7.4 by PAMPA assay		3	ALA4842384	assay format	Scientific Literature
17.	ALA4845062	A	Cmax in C57BL6 mouse at 30 mg/kg, po	Mus musculus	3	ALA4842384	organism-based format	Scientific Literature
18.	ALA4845063	A	AUC in C57BL6 mouse at 30 mg/kg, po	Mus musculus	3	ALA4842384	organism-based format	Scientific Literature
19.	ALA4845064	A	Oral bioavailability in C57BL6 mouse at 30 mg/kg	Mus musculus	3	ALA4842384	organism-based format	Scientific Literature
20.	ALA4845065	B	Inhibition of human MGAT3	Homo sapiens	3	ALA4842384	single protein format	Scientific Literature