| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3861312 | F | Immunosuppressive activity in human anti-CD3/anti-CD28 stimulated Jurkat T cells assessed as suppression of IL2 production at 10 uM pretreated with cells followed by anti-CD3/CD28 stimulation measured after 13 hrs by ELISA | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 2. | ALA3861314 | F | Immunosuppressive activity in human PMA/ionomycin stimulated Jurkat T cells assessed as suppression of IL2 production pretreated with cells followed by PMA/ionomycin stimulation measured after 13 hrs by ELISA | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 3. | ALA3861317 | B | Inhibition of ERK phosphorylation in human anti-CD3/CD28 stimulated Jurkat T cells at 10 uM pretreated with cells followed by anti-CD3/CD28 stimulation measured up to 60 mins immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA3861318 | B | Inhibition of JNK phosphorylation in human anti-CD3/anti-CD28 stimulated Jurkat T cells at 10 uM pretreated with cells followed by anti-CD3/CD28 stimulation measured up to 60 mins immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA3861319 | B | Inhibition of IkappaBalpha phosphorylation in human anti-CD3/anti-CD28 stimulated Jurkat T cells at 10 uM pretreated with cells followed by anti-CD3/anti-CD28 stimulation measured up to 60 mins immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA3861320 | B | Reduction in NFATc1 nuclear localization in human anti-CD3/anti-CD28 stimulated Jurkat T cells at 10 ug measured after 15 mins by SDS-PAGE and immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA3861321 | B | Inhibition of NFkappaB activation in human anti-CD3/anti-CD28 stimulated Jurkat T cells at 10 uM measured by renilla luciferase reporter gene assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3861322 | B | Inhibition of AP-1 activation in human PMA/ionomycin stimulated Jurkat T cells at 10 uM by renilla luciferase reporter gene assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA3861323 | T | Cytotoxicity against human Jurkat T cells assessed as cell survival at 10 uM measured after 3 days by MTT assay | Homo sapiens | 33 | cell-based format | Scientific Literature | ||
| 10. | ALA3861324 | B | Inhibition of recombinant full length human N-terminal His6-tagged IKKbeta expressed in baculovirus infected sf21 cells at 10 uM using IKKtide as substrate after 40 mins in presence of [gamma33P]ATP by scintillation counting method relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA3861325 | A | Inhibition of recombinant human CYP3A4 expressed in baculosomes at 10 uM preincubated with protein prior to substrate addition measured after 40 mins in presence of NADP regeneration system by fluorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA3861326 | A | Inhibition of recombinant human CYP2C9 expressed in baculosomes at 10 uM preincubated with protein prior to substrate addition measured after 40 mins in presence of NADP regeneration system by fluorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA3861327 | A | Inhibition of recombinant human CYP1A2 expressed in baculosomes at 10 uM preincubated with protein prior to substrate addition measured after 40 mins in presence of NADP regeneration system by fluorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA3861328 | A | Inhibition of recombinant human CYP3A4 expressed in baculosomes at 1 uM preincubated with protein prior to substrate addition measured after 40 mins in presence of NADP regeneration system by fluorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA3861329 | A | Inhibition of recombinant human CYP2C9 expressed in baculosomes at 1 uM preincubated with protein prior to substrate addition measured after 40 mins in presence of NADP regeneration system by fluorimetric analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 16. | ALA3861330 | B | Inhibition of recombinant full length human N-terminal His6-tagged IKKbeta expressed in baculovirus infected sf21 cells at 1 uM using IKKtide as substrate after 40 mins in presence of [gamma33P]ATP by scintillation counting method relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA3861331 | A | Half life in human liver S9 fraction at 5 uM in presence of NADPH by LC-MS/MS analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 18. | ALA3861332 | A | Half life in rat liver microsome S9 fraction at 5 uM in presence of NADPH by LC-MS/MS analysis | Rattus norvegicus | 1 | subcellular format | Scientific Literature | ||
| 19. | ALA3861333 | A | Half life in mouse liver microsome S9 fraction at 5 uM in presence of NADPH by LC-MS/MS analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 20. | ALA3861334 | B | Inhibition of Kv1.3 channel (unknown origin) expressed in 293 cells assessed as inhibition of potassium flux at 10 uM preincubated for 30 mins followed by stimulation with thallium for 90 mins by thallium-sensitive FluxOR-dye based fluorescence analysis | Homo sapiens | 5 | cell-based format | Scientific Literature |
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