Antagonist activity at recombinant human mGlu5 receptor expressed in hamster AV12 cells expressing EAAT1 assessed as inhibition of glutamate-induced intracellular Ca2+ level measured after 24 hrs by FLIPR assay
Positive allosteric modulation of recombinant human mGlu2 receptor expressed in hamster AV12 cells expressing EAAT1 assessed as potentiation of glutamate-induced intracellular calcium level measured after 24 to 48 hrs by FLIPR assay
Positive allosteric modulation of recombinant human mGlu2 receptor expressed in hamster AV12 cells expressing EAAT1 assessed as potentiation of glutamate-induced intracellular calcium level measured after 24 to 48 hrs by FLIPR assay relative to glutamate
Antagonist activity at human CysLT1 receptor expressed in Syrian hamster AV12-664 cells assessed as decrease in LTD4-induced intracellular calcium level pretreated followed by LTD4 addition by fluo-3 dye based FLIPR assay
Decrease in plasma protein extravasation in fasted Harlan Sprague-Dawley rat model of migraine compound administered via oral gavage 1 hr prior to electrical stimulation of trigeminal ganglion measured after 15 mins by FITC-BSA based fluorescence assay