Displacement of [125I]-p-iodoclonidine from I1 receptor imidazoline binding site in Sprague-Dawley rat kidney cell membranes after 30 mins by liquid scintillation counting
Displacement of [3H]-2-BFI from I2 receptor imidazoline binding site in Sprague-Dawley rat brain membranes after 30 mins by liquid scintillation counting
Agonist activity at human alpha2b-AR expressed in CHO cells assessed as rate of acidification after 240 mins by cytosensor microphysiometric analysis relative to (-)-noradrenaline
Agonist activity at human alpha2c-AR expressed in CHO cells assessed as rate of acidification after 240 mins by cytosensor microphysiometric analysis relative to (-)-noradrenaline
Antagonist activity at human alpha2a-AR expressed in CHO cells assessed as inhibition of (-)-noradrenaline-induced acidification rate after 240 mins by cytosensor microphysiometric analysis
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated jumping at 5 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated jumping at 10 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated rearing frequency at 5 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated forepaw tremor frequency at 5 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated teeth chattering frequency at 5 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated rearing frequency at 10 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated forepaw tremor frequency at 10 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence expression in CD1 mouse assessed as inhibition of naloxone-precipitated teeth chattering frequency at 10 mg/kg, ip administered acutely 15 mins prior to naloxone challenge relative to control
Reduction in morphine dependence acquisition in CD1 mouse assessed as inhibition of naloxone-precipitated jumping at 5 to 10 mg/kg, ip administered 15 mins prior to each morphine challenge relative to control
Reduction in morphine dependence acquisition in CD1 mouse assessed as inhibition of naloxone-precipitated rearing frequency at 5 to 10 mg/kg, ip administered 15 mins prior to each morphine challenge relative to control
Reduction in morphine dependence acquisition in CD1 mouse assessed as inhibition of naloxone-precipitated forepaw tremor frequency at 5 to 10 mg/kg, ip administered 15 mins prior to each morphine challenge relative to control
Reduction in morphine dependence acquisition in CD1 mouse assessed as inhibition of naloxone-precipitated teeth chattering frequency at 5 to 10 mg/kg, ip administered 15 mins prior to each morphine challenge relative to control