| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3870775 | B | Inhibition of recombinant His-tagged Cyp18 (unknown origin) expressed in Escherichia coli BL21(DE3) up to 1000 nM | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 2. | ALA3870776 | B | Binding affinity to recombinant His-tagged Cyp18 (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as inhibition of recombinant human His6-tagged calcineurin alpha phosphatase actvity using RII phosphopeptide as substrate by measuring amount of free phosphate at 10 uM preincubated for 20 mins followed by substrate addition measured after 30 mins in presence of human calmodulin by malachite green dye-based spectrophotometry relative to control | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 3. | ALA3870777 | B | Inhibition of recombinant 6His-tagged human calcineurin-alpha expressed in Escherichia coli BL21(DE3/pLysS) assessed as inhibition of calcineurin phosphatase activity by measuring amount of free phosphate at 10 uM preincubated for 20 mins followed by addition of bovine RII phosphopeptide as substrate measured after 30 mins in presence of human calmodulin by malachite green dye-based spectrophotometry relative to DMSO control | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 4. | ALA3870778 | B | Binding affinity to recombinant His-tagged Cyp18 (unknown origin) expressed in Escherichia coli BL21(DE3) by fluorescence analysis | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 5. | ALA3870779 | B | Binding affinity to recombinant His-tagged Cyp18 (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as dissociation rate constant in presence of 20-fold excess of cyclosporin A by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA3870781 | B | Binding affinity to Cyp18 (unknown origin) assessed as association rate constant of fast phase by stopped flow method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA3870782 | B | Binding affinity to Cyp18 (unknown origin) by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA3870788 | B | Binding affinity to recombinant His-tagged Cyp18 (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as blue shift by UV absorbance method | Homo sapiens | 4 | assay format | Scientific Literature |
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