# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3879162 | B | Inhibition of full length recombinant human DAGLalpha expressed in HEK293T cell membranes using PNP butyrate as substrate by colorimetric assay | Homo sapiens | 31 | ALA3877306 | cell-based format | Scientific Literature | |
2. | ALA3879167 | B | Inhibition of human ABHD6 using 2-AG as natural substrate by fluorescence based assay | Homo sapiens | 27 | ALA3877306 | single protein format | Scientific Literature | |
3. | ALA3879168 | B | Inhibition of recombinant human DAGLalpha expressed in HEK293T cell membranes overexpressing MAGL using natural substrate 1-stearoyl-2-arachidonoly-sn-glycerol by fluorescence based assay | Homo sapiens | 3 | ALA3877306 | cell-based format | Scientific Literature | |
4. | ALA3879169 | B | Inhibition of MB064 probe binding to DAGLalpha in mouse brain membrane proteome preincubated for 30 mins followed by MB064 probe addition measured after 20 mins by gel-based competitive activity based protein profiling assay | Mus musculus | 7 | ALA3877306 | cell membrane format | Scientific Literature | |
5. | ALA4262256 | B | Inhibition of recombinant human DAGLalpha expressed in HEK293 cells using PNP-butyrate as substrate pretreated for 20 to 30 mins followed by substrate addition measured every 60 secs for 20 mins by colorimetric method | Homo sapiens | 23 | ALA4261499 | cell-based format | Scientific Literature | |
6. | ALA4262257 | B | Inhibition of recombinant human ABHD6 expressed in HEK293 cells by AmplifuTM red dye based fluorescence assay | Homo sapiens | 23 | ALA4261499 | cell-based format | Scientific Literature | |
7. | ALA4262258 | B | Inhibition of MB064 labelling of DAGLalpha in mouse brain homogenates at 10 uM pretreated for 30 mins followed by MB064 addition and measured after 20 mins by activity based protein profiling assay relative to control | Mus musculus | 22 | ALA4261499 | tissue-based format | Scientific Literature | |
8. | ALA4262259 | B | Inhibition of MB064 labelling of ABHD6 in mouse brain homogenates at 10 uM pretreated for 30 mins followed by MB064 addition and measured after 20 mins by activity based protein profiling assay relative to control | Mus musculus | 22 | ALA4261499 | tissue-based format | Scientific Literature | |
9. | ALA5234403 | B | Inhibition of human recombinant ABHD6 | Homo sapiens | 5 | ALA5233244 | single protein format | Scientific Literature | |
10. | ALA5234404 | B | Inhibition of ABHD6 in mouse brain membrane using TAMRA-FP as substrate at 10 uM pre-incubated for 30 mins followed by substrate addition and measured after 20 mins by coomassie staining based gel competitive ABPP assay | Mus musculus | 2 | ALA5233244 | cell membrane format | Scientific Literature |