# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
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1. | ALA3887026 | Binding | qHTS Assay: Assay details and protocol: The primary assay monitored ATP depletion using Promega's KinaseGlo technology, where ATP levels are measured through luminescence generated from firefly luciferase, a bioluminescent ATP-dependent enzymes. ATP was held at 35 μM, near its reported KM value, and the KM for galactose was determined under the 1536-well assay conditions to be 50-100M (FIG. 1A). As well, the IC50 for a commercially available CD45 inhibitor (N-(9,10-dioxo-9,10-dihydrophenanthren-2-yl)pivalamide) was confirmed (previously found to inhibit GALK (FIG. 1B)). This was used as the positive control for the assay. The assay used 5 nM GALK and a 1 hr incubation time, which gave sufficient signal:background and stability for the HTS. | 88 | ALA3886142 | single protein format | BindingDB Database | ||
2. | ALA5052897 | Binding | Inhibition of GALK (unknown origin) incubated for 1 hr and measured by qHTS assay | Homo sapiens | 91 | ALA5052821 | single protein format | Patent Bioactivity Data | |
3. | ALA5052898 | Binding | Inhibition of GALK (unknown origin) incubated for 1 hr and measured by qHTS assay realtive to control | Homo sapiens | 91 | ALA5052821 | single protein format | Patent Bioactivity Data | |
4. | ALA5052899 | Binding | Inhibition of bacterial CDP-ME kinase assessed as ATP depletion up to 57 uM measured by bioluminescent kinase Glo assay | Bacteria | 91 | ALA5052821 | assay format | Patent Bioactivity Data |