#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3854930	Binding	Inhibition of human COT (66 to 395 residues) expressed in Sf21 cells using 5-Fluo-Ahx-AGAGSGQLIDSNleANSFVGTR-NH2 as substrate after 60 mins by caliper microfluidic mobility shift assay	Homo sapiens	25	ALA3853320	cell-based format	Scientific Literature
2.	ALA3854931	Binding	Inhibition of MEK/ERK signaling pathway in TNFalpha-stimulated human A431 cells transfected with COT siRNA assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by stimulation with TNFalpha for 10 mins by Western blot analysis	Homo sapiens	2	ALA3853320	cell-based format	Scientific Literature
3.	ALA3854932	Binding	Inhibition of MEK/ERK signaling pathway in EGF-stimulated human A431 cells transfected with COT siRNA assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by stimulation with EGF for 10 mins by Western blot analysis	Homo sapiens	2	ALA3853320	cell-based format	Scientific Literature
4.	ALA3854933	Binding	Inhibition of COT in LPS-stimulated human PBMC assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by LPS stimulation for 12 mins by flow cytometric analysis	Homo sapiens	8	ALA3853320	single protein format	Scientific Literature
5.	ALA3854934	Binding	Inhibition of COT in LPS-stimulated human PBMC assessed as reduction in TNFalpha release incubated for 1 hr followed by stimulation with LPS for 4 hrs by HTRF assay	Homo sapiens	8	ALA3853320	single protein format	Scientific Literature
6.	ALA3854935	Binding	Inhibition of NFkappaB pathway in LPS-stimulated human PBMC assessed as reduction in p65 phosphorylation incubated for 60 mins followed by LPS stimulation for 12 mins by flow cytometric analysis analysis	Homo sapiens	1	ALA3853320	assay format	Scientific Literature
7.	ALA3854936	Binding	Inhibition of FLT3 (563 to 993 residues) D835Y mutant (unknown origin) after 60 mins in presence of ATP by caliper microfluidic mobility shift assay	Homo sapiens	1	ALA3853320	assay format	Scientific Literature
8.	ALA3854937	Binding	Inhibition of MKNK2 (unknown origin) after 60 mins in presence of ATP by caliper microfluidic mobility shift assay	Homo sapiens	1	ALA3853320	single protein format	Scientific Literature
9.	ALA3854938	Physicochemical	Octanol-water partition coefficient, log P of the compound by shake-flask method		11	ALA3853320	small-molecule physicochemical format	Scientific Literature
10.	ALA3854940	Binding	Inhibition of COT in LPS-stimulated human whole blood assessed as reduction in TNFalpha release incubated for 30 mins followed by stimulation with LPS for 3 hrs by HTRF assay	Homo sapiens	7	ALA3853320	tissue-based format	Scientific Literature
11.	ALA3854941	Physicochemical	Equilibrium solubility of the compound at pH 6.8 after 16 hrs by mass spectrometric analysis		9	ALA3853320	small-molecule physicochemical format	Scientific Literature
12.	ALA3854942	Physicochemical	Equilibrium solubility of the compound at pH 4 after 16 hrs by mass spectrometric analysis		9	ALA3853320	small-molecule physicochemical format	Scientific Literature
13.	ALA3854943	ADME	Protein binding in human plasma at 5 uM after 4 hrs by rapid equilibrium dialysis method	Homo sapiens	6	ALA3853320	cell-free format	Scientific Literature