#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888333	Binding	SPR Competitive Binding Assay: SPR interaction analyses were performed with a Biacore 2000, using Biacore 2000 Control Software v3.2 and BIAevaluation v4.1 analysis software (Biacore) as described in Mol Cancer Ther 7:2621 (2008). For the competitive binding assays and the Ki determination, PtdIns(3,4,5)phosphate-biotin labeled liposomes (Echelon Biosciences) and SA chips were used with increasing concentrations of the compound tested.		32	ALA3886664	single protein format	BindingDB Database
2.	ALA3888334	Binding	Cellular Assay: Inhibition of AKT was measured by Western blots using specific antibodies against phospho-Ser473-AKT in HT-29 lung cancer cells. Inhibition of the phosphorylation of AKT and its downstream targets was measured by Western blotting using rabbit polyclonal antibodies to phospho-Ser473-AKT, phospho-Thr308-AKT, total-AKT, phospho-Ser9-GSK3β, phospho-Ser21-GSK3β, phospho-Ser241-PDK1 and phospho-Thr389p70S6-kinase (New England Biolabs/Cell Signaling Technology Inc.) using β-Actin as a loading control as described in Mol Cancer Ther 7:2621 (2008). Bands corresponding to phospho-Ser473-AKT and total AKT were quantified using Eagle Eye software (BioRad) and Kodak X-Omat™ Blue XB (NEN™, Life Science Products). Cell growth inhibition was determined using a microcytotoxicity assay and apoptosis was measured as described in Mol Cancer Ther 7:2621 (2008).		9	ALA3886664	cell-based format	BindingDB Database
3.	ALA4482511	Binding	Binding affinity to recombinant human GAB1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay	Homo sapiens	22	ALA4482310	assay format	Patent Bioactivity Data
4.	ALA4482512	Binding	Binding affinity to recombinant human IRS1 PH domain expressed in Escherichia coli BL21 (DE3) by surface plasmon resonance assay	Homo sapiens	22	ALA4482310	assay format	Patent Bioactivity Data
5.	ALA4482513	Binding	Binding affinity to AKT1 (unknown origin) by surface plasmon resonance assay	Homo sapiens	22	ALA4482310	single protein format	Patent Bioactivity Data
6.	ALA4482514	Binding	Inhibition of HGF-stimulated GAB1 Y627 phosphorylation in human T47D cells at 20 uM pre-incubated for 4 hrs before stimulation with HGF for 20 mins	Homo sapiens	22	ALA4482310	cell-based format	Patent Bioactivity Data
7.	ALA4482515	Functional	Cytotoxicity in human MDA-MB-231 cells assessed as reduction in cell viability incubated for 4 days by MTT assay	Homo sapiens	22	ALA4482310	cell-based format	Patent Bioactivity Data
8.	ALA4482516	Functional	Cytotoxicity in human T47D cells assessed as reduction in cell viability incubated for 4 days by MTT assay	Homo sapiens	22	ALA4482310	cell-based format	Patent Bioactivity Data
9.	ALA4482518	Binding	Inhibition of HGF-induced IRS1 phosphorylation in human T47D cells assessed as pSer312 IRS1/total-IRS1 ratio level at 20 uM pre-incubated for 4 hrs before stimulation with HGF for 20 mins (Rvb = 100 +/- 0.02%)	Homo sapiens	21	ALA4482310	cell-based format	Patent Bioactivity Data
10.	ALA4482520	ADME	Cytotoxicity in human MCF10A cells assessed as reduction in cell viability at 100 uM incubated for 4 days by MTT assay	Homo sapiens	2	ALA4482310	cell-based format	Patent Bioactivity Data