#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3887676	B	Thallium Flux Assay: The ROMK channel functional thallium flux assay is performed in 384 wells, using the FLIPR-Tetra instrument. HEK-hKir1.1 cells are seeded in Poly-D-Lysine microplates and kept in a 37° C.-10% CO2 incubator overnight. On the day of the experiment, the growth media is replaced with the FluxOR reagent loading buffer and incubated, protected from light, at ambient temperature (23-25° C.) for 90 min. The loading buffer is replaced with assay buffer+/-test compound followed by 30 min incubation at ambient temperature, where the Thallium/Potassium stimulant is added to the microplate.Step Protocol 1. Seed HEK-hKir1.1 cells (50 uA at 20,000 cells/well) in 384-well PDL coated Microplates 2. Allow cells to adhere overnight in humidified 37° C./10% CO2 incubator 3. Completely remove cell growth media from microplate and replace with 25 ul loading buffer 4. Incubate Microplate at room temperature, protected form light, for 90 min.	Homo sapiens	76		assay format	BindingDB Database
2.	ALA3887677	B	Electrophysiology Assay: Block of Kir1.1 (ROMK1) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391:85-100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, Calif.). Chinese hamster ovary cells stably expressing Kir1.1 channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37° C. Prior to an experiment, Kir1.1 expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 mL of Versene (Invitrogen 15040-066) for approximately 6 min at 37° C. and suspended in 10 mL of bath solution containing (in mM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES, pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 mL of bath solution and placed in the IonWorks instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF, 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4.	Homo sapiens	29		cell-based format	BindingDB Database
3.	ALA5039907	B	Inhibition of human ROMK expressed in CHO cells by whole cell voltage clamp electrophysiology assay	Homo sapiens	14		cell-based format	Scientific Literature
4.	ALA5039908	T	Inhibition of human ERG expressed in HEK-293 cells by voltage clamp electrophysiology assay	Homo sapiens	14		cell-based format	Scientific Literature
5.	ALA5039910	A	Apparent permeability in pig LLC-PK1 cells	Sus scrofa	12		cell-based format	Scientific Literature
6.	ALA5039911	B	Inhibition of rat Kir1.1 expressed in HEK293 cells by electrophysiology assay	Rattus norvegicus	1		cell-based format	Scientific Literature
7.	ALA5039912	A	Clearance in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
8.	ALA5039913	A	Volume of distribution at steady state in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
9.	ALA5039914	A	Effective half life in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
10.	ALA5039915	A	Terminal half life in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
11.	ALA5039916	A	Cmax in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
12.	ALA5039917	A	Tmax in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
13.	ALA5039918	A	AUC (0 to infinity) in Wistar Han rat at 2 mg/kg, po or 1 mg/kg, iv	Rattus norvegicus	1		organism-based format	Scientific Literature
14.	ALA5039919	A	Oral bioavailability in Wistar Han rat at 2 mg/kg	Rattus norvegicus	1		organism-based format	Scientific Literature
15.	ALA5039920	A	Clearance in Beagle dog at 2 mg/kg, po or 1 mg/kg, iv	Canis lupus familiaris	1		organism-based format	Scientific Literature
16.	ALA5039921	A	Volume of distribution at steady state in Beagle dog at 2 mg/kg, po or 1 mg/kg, iv	Canis lupus familiaris	1		organism-based format	Scientific Literature
17.	ALA5039922	A	Effective half life in Beagle dog at 2 mg/kg, po or 1 mg/kg, iv	Canis lupus familiaris	1		organism-based format	Scientific Literature
18.	ALA5039923	A	Terminal half life in Beagle dog at 2 mg/kg, po or 1 mg/kg, iv	Canis lupus familiaris	1		organism-based format	Scientific Literature
19.	ALA5039924	A	Cmax in Beagle dog at 2 mg/kg, po or 1 mg/kg, iv	Canis lupus familiaris	1		organism-based format	Scientific Literature
20.	ALA5039925	A	Tmax in Beagle dog at 2 mg/kg, po or 1 mg/kg, iv	Canis lupus familiaris	1		organism-based format	Scientific Literature