#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888092	B	Cathepsin Enzyme Inhibition Assay: Enzyme activity is measured by observing the increase in fluorescence intensity caused by cleavage of a peptide substrate containing a fluorophore whose emission is quenched in the intact peptide. Assay buffer: 100 mM potassium phosphate pH 6.5, EDTA-Na 5 mM, Triton X-100 0.001%, DDT 5 mM. Enzymes (all at 1 nM): human and mouse Cathepsin S, Cat K, Cat B, Cat LSubstrate (20 uM): Z-Val-Val-Arg-AMC, except for Cat K which uses Z-Leu-Arg-AMC (both from Bachem). Z=Benzyloxycarbonyl. AMC=7-Amino-4-Methyl-Coumarin. Final volume: 100 uL. Excitation 360 nm, Emission 465 nm. Enzyme is added to the substance dilutions in 96-well microtitre plates and the reaction is started with substrate. Fluorescence emission is measured over 20 minutes, during which time a linear increase is observed in the absence of inhibitor.	Homo sapiens	49		single protein format	BindingDB Database
2.	ALA4049053	P	Octanol-water intrinsic distribution co-efficient, log D of the compound at 100 uM at pH 7.4 after 16 to 22 hrs by CAMDIS assay		1		small-molecule physicochemical format	Scientific Literature
3.	ALA4049054	P	Solubility of the compound at pH 6.5 by LYSA		1		small-molecule physicochemical format	Scientific Literature
4.	ALA4049055	B	Inhibition of Trypanosoma brucei rhodesiense rhodesain using Cbz-Phe-Arg-AMC as substrate by fluorimetric method	Trypanosoma brucei rhodesiense	25		single protein format	Scientific Literature
5.	ALA4049056	B	Inhibition of human CatL using Cbz-Phe-Arg-AMC as substrate measured over 30 mins by fluorimetric method	Homo sapiens	26		single protein format	Scientific Literature
6.	ALA4049060	P	Effective permeability of the compound after 15 hrs by PAMPA		1		small-molecule physicochemical format	Scientific Literature
7.	ALA4049061	A	Inhibition of CYP3A4 in human liver microsomes using DBF as substrate by fluorescence assay	Homo sapiens	1		assay format	Scientific Literature
8.	ALA4049062	A	Inhibition of CYP2D6 in human liver microsomes using AMMC as substrate by fluorescence assay	Homo sapiens	1		assay format	Scientific Literature
9.	ALA4049063	A	Inhibition of CYP2C9 in human liver microsomes using MFC as substrate by fluorescence assay	Homo sapiens	1		assay format	Scientific Literature
10.	ALA4049064	A	Half life in C57BL/6J mouse administered via iv as bolus dose by LC-MS/MS analysis	Mus musculus	1		organism-based format	Scientific Literature
11.	ALA4049065	A	Clearance in C57BL/6J mouse administered via iv as bolus dose by LC-MS/MS analysis	Mus musculus	1		organism-based format	Scientific Literature
12.	ALA4049066	A	Volume of distribution at steady state in C57BL/6J mouse administered via iv as bolus dose by LC-MS/MS analysis	Mus musculus	1		organism-based format	Scientific Literature
13.	ALA4049067	A	Ratio of drug level in brain to plasma in C57BL/6J mouse administered via iv as bolus dose by LC-MS/MS analysis	Mus musculus	1		organism-based format	Scientific Literature
14.	ALA4049068	A	Efflux ratio of permeability across pig LLC-PK1 cells expressing human MDR1 at 1 uM after 3.5 hrs by LC-MS/MS analysis	Sus scrofa	2		cell-based format	Scientific Literature
15.	ALA4049069	A	Efflux ratio of permeability across pig LLC-PK1 cells expressing mouse Mdr1a at 1 uM after 3.5 hrs by LC-MS/MS analysis	Sus scrofa	2		cell-based format	Scientific Literature
16.	ALA4049070	F	Antitrypanosomal activity against bloodstream form of Trypanosoma brucei rhodesiense STIB 900 infected in NMRI mouse assessed as reduction of parasitemia at 25 mg/kg, ip bid administered for 4 consecutive days starting 3 days post infection measured 24 hrs post last dose	Trypanosoma brucei rhodesiense	2		organism-based format	Scientific Literature
17.	ALA4049072	A	Plasma concentration in rat at 3 mg/kg administered via infusion over 30 mins	Rattus norvegicus	1		organism-based format	Scientific Literature
18.	ALA4049073	A	Drug uptake in rat brain at 3 mg/kg administered via infusion over 30 mins	Rattus norvegicus	1		organism-based format	Scientific Literature
19.	ALA4049074	A	Drug uptake in rat cerebrospinal fluid at 3 mg/kg administered via infusion over 30 mins	Rattus norvegicus	1		organism-based format	Scientific Literature
20.	ALA4049075	A	Ratio of drug level in rat brain to plasma at 3 mg/kg administered via infusion over 30 mins	Rattus norvegicus	1		organism-based format	Scientific Literature