#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3854930	Binding	Inhibition of human COT (66 to 395 residues) expressed in Sf21 cells using 5-Fluo-Ahx-AGAGSGQLIDSNleANSFVGTR-NH2 as substrate after 60 mins by caliper microfluidic mobility shift assay	Homo sapiens	25	ALA3853320	cell-based format	Scientific Literature
2.	ALA3854933	Binding	Inhibition of COT in LPS-stimulated human PBMC assessed as reduction in ERK1/2 phosphorylation incubated for 60 mins followed by LPS stimulation for 12 mins by flow cytometric analysis	Homo sapiens	8	ALA3853320	single protein format	Scientific Literature
3.	ALA3854934	Binding	Inhibition of COT in LPS-stimulated human PBMC assessed as reduction in TNFalpha release incubated for 1 hr followed by stimulation with LPS for 4 hrs by HTRF assay	Homo sapiens	8	ALA3853320	single protein format	Scientific Literature
4.	ALA3854938	Physicochemical	Octanol-water partition coefficient, log P of the compound by shake-flask method		11	ALA3853320	small-molecule physicochemical format	Scientific Literature
5.	ALA3854939	ADME	Clearance in wild type Sprague-Dawley rat plasma at 1 mg/kg, iv	Rattus norvegicus	3	ALA3853320	organism-based format	Scientific Literature
6.	ALA3854940	Binding	Inhibition of COT in LPS-stimulated human whole blood assessed as reduction in TNFalpha release incubated for 30 mins followed by stimulation with LPS for 3 hrs by HTRF assay	Homo sapiens	7	ALA3853320	tissue-based format	Scientific Literature
7.	ALA3854941	Physicochemical	Equilibrium solubility of the compound at pH 6.8 after 16 hrs by mass spectrometric analysis		9	ALA3853320	small-molecule physicochemical format	Scientific Literature
8.	ALA3854942	Physicochemical	Equilibrium solubility of the compound at pH 4 after 16 hrs by mass spectrometric analysis		9	ALA3853320	small-molecule physicochemical format	Scientific Literature
9.	ALA3854943	ADME	Protein binding in human plasma at 5 uM after 4 hrs by rapid equilibrium dialysis method	Homo sapiens	6	ALA3853320	cell-free format	Scientific Literature
10.	ALA3854944	ADME	Half life in wild type Sprague-Dawley rat liver microsomes at 5 uM preincubated for 3 mins followed by NADPH addition measured after 1 hr by HPLC/MS analysis	Rattus norvegicus	3	ALA3853320	tissue-based format	Scientific Literature
11.	ALA3854983	ADME	Metabolic stability in Sprague-Dawley rat liver microsomes assessed as parent compound remaining at 5 uM after 1 hr in presence of NADPH by HPLC/MS analysis	Rattus norvegicus	2	ALA3853320	tissue-based format	Scientific Literature
12.	ALA3854984	ADME	Metabolic stability in bile-duct cannulated Sprague-Dawley rat assessed as parent compound remaining in blood at 3 mg/kg, iv measured 1 hr post dose	Rattus norvegicus	2	ALA3853320	organism-based format	Scientific Literature
13.	ALA3854985	ADME	Metabolic stability in bile-duct cannulated Sprague-Dawley rat assessed as parent compound remaining in blood at 3 mg/kg, iv measured 4 hrs post dose	Rattus norvegicus	2	ALA3853320	organism-based format	Scientific Literature
14.	ALA3854986	ADME	Metabolic stability in bile-duct cannulated Sprague-Dawley rat assessed as parent compound remaining in bile at 3 mg/kg, iv measured over 8 hrs post dose	Rattus norvegicus	2	ALA3853320	organism-based format	Scientific Literature
15.	ALA3854987	ADME	Metabolic stability in bile-duct cannulated Sprague-Dawley rat assessed as parent compound remaining in urine at 3 mg/kg, iv measured over 8 hrs post dose	Rattus norvegicus	2	ALA3853320	organism-based format	Scientific Literature
16.	ALA3854988	ADME	Drug excretion in bile-duct cannulated Sprague-Dawley rat bile at 3 mg/kg, iv	Rattus norvegicus	2	ALA3853320	organism-based format	Scientific Literature
17.	ALA3854989	ADME	Drug excretion in bile-duct cannulated Sprague-Dawley rat urine at 3 mg/kg, iv	Rattus norvegicus	2	ALA3853320	organism-based format	Scientific Literature