#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3887812	B	In Vitro Inhibition Assay: Plasma kallikrein inhibitory activity in vitro was determined using standard published methods (see e.g. Johansen et al., Int. J. Tiss. Reac. 1986, 8, 185; Shori et al., Biochem. Pharmacol., 1992, 43, 1209; Stiirzebecher et al., Biol. Chem. Hoppe-Seyler, 1992, 373, 1025). Human plasma kallikrein (Protogen) was incubated at 37° C. with the fluorogenic substrate H-DPro-Phe-Arg-AFC and various concentrations of the test compound. Residual enzyme activity (initial rate of reaction) was determined by measuring the change in optical absorbance at 410 nm and the IC50 value for the test compound was determined.	Homo sapiens	198		cell-free format	BindingDB Database
2.	ALA3887813	B	In Vitro Inhibition Assay: KLK1 inhibitory activity in vitro was determined using standard published methods (see e.g. Johansen et al., Int. J. Tiss. Reac. 1986, 8, 185; Shori et al., Biochem. Pharmacol., 1992, 43, 1209; Stürzebecher et al., Biol. Chem. Hoppe-Seyler, 1992, 373, 1025). Human KLK1 (Callbiochem) was incubated at 37° C. with the fluorogenic substrate H-DVal-Leu-Arg-AFC and various concentrations of the test compound. Residual enzyme activity (initial rate of reaction) was determined by measuring the change in optical absorbance at 410 nm and the IC50 value for the test compound was determined.		181		single protein format	BindingDB Database
3.	ALA3887814	B	Enzyme Assay: Human serine protease enzymes plasmin, thrombin, trypsin, Factor Xa and Factor XIIa were assayed for enzymatic activity using an appropriate fluorogenic substrate. Protease activity was measured by monitoring the accumulation of liberated fluorescence from the substrate over 5 minutes. The linear rate of fluorescence increase per minute was expressed as percentage (%) activity. The Km for the cleavage of each substrate was determined by standard transformation of the Michaelis-Menten equation.	Homo sapiens	1		single protein format	BindingDB Database
4.	ALA3887815	B	Enzyme Assay: Human serine protease enzymes plasmin, thrombin, trypsin, Factor Xa and Factor XIIa were assayed for enzymatic activity using an appropriate fluorogenic substrate. Protease activity was measured by monitoring the accumulation of liberated fluorescence from the substrate over 5 minutes. The linear rate of fluorescence increase per minute was expressed as percentage (%) activity. The Km for the cleavage of each substrate was determined by standard transformation of the Michaelis-Menten equation.	Homo sapiens	1		single protein format	BindingDB Database
5.	ALA3887816	B	Enzyme Assay: Human serine protease enzymes plasmin, thrombin, trypsin, Factor Xa and Factor XIIa were assayed for enzymatic activity using an appropriate fluorogenic substrate. Protease activity was measured by monitoring the accumulation of liberated fluorescence from the substrate over 5 minutes. The linear rate of fluorescence increase per minute was expressed as percentage (%) activity. The Km for the cleavage of each substrate was determined by standard transformation of the Michaelis-Menten equation.	Homo sapiens	1		single protein format	BindingDB Database
6.	ALA3887817	B	Enzyme Assay: Human serine protease enzymes plasmin, thrombin, trypsin, Factor Xa and Factor XIIa were assayed for enzymatic activity using an appropriate fluorogenic substrate. Protease activity was measured by monitoring the accumulation of liberated fluorescence from the substrate over 5 minutes. The linear rate of fluorescence increase per minute was expressed as percentage (%) activity. The Km for the cleavage of each substrate was determined by standard transformation of the Michaelis-Menten equation.	Homo sapiens	1		single protein format	BindingDB Database
7.	ALA3887818	B	Enzyme Assay: Human serine protease enzymes plasmin, thrombin, trypsin, Factor Xa and Factor XIIa were assayed for enzymatic activity using an appropriate fluorogenic substrate. Protease activity was measured by monitoring the accumulation of liberated fluorescence from the substrate over 5 minutes. The linear rate of fluorescence increase per minute was expressed as percentage (%) activity. The Km for the cleavage of each substrate was determined by standard transformation of the Michaelis-Menten equation.		26		single protein format	BindingDB Database
8.	ALA5233998	B	Inhibition of plasma kallikrein (unknown origin)	Homo sapiens	17		single protein format	Scientific Literature
9.	ALA5233999	B	Inhibition of plasmin (unknown origin)	Homo sapiens	8		single protein format	Scientific Literature
10.	ALA5234000	B	Inhibition of thrombin (unknown origin)	Homo sapiens	5		single protein format	Scientific Literature
11.	ALA5234003	B	Inhibition of fXIIa (unknown origin)	Homo sapiens	2		single protein format	Scientific Literature
12.	ALA5234020	B	Inhibition of human plasma kallikrein using H-DPro-Phe-Arg-AFC as substrate	Homo sapiens	8		single protein format	Scientific Literature
13.	ALA5234025	B	Inhibition of human KLK1 using H-DVal-Leu-Arg-AFC as substrate	Homo sapiens	5		single protein format	Scientific Literature
14.	ALA5234026	B	Inhibition of human plasmin by fluorescence based analysis	Homo sapiens	1		single protein format	Scientific Literature
15.	ALA5234027	B	Inhibition of human thrombin by fluorescence based analysis	Homo sapiens	1		single protein format	Scientific Literature
16.	ALA5234028	B	Inhibition of human trypsin by fluorescence based analysis	Homo sapiens	1		protein format	Scientific Literature
17.	ALA5234029	B	Inhibition of human fXA by fluorescence based analysis	Homo sapiens	1		single protein format	Scientific Literature
18.	ALA5234030	B	Inhibition of human fXIIA by fluorescence based analysis	Homo sapiens	1		single protein format	Scientific Literature