# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3863727 | Physicochemical | Induction of reactive oxygen species in human MDA-MB-231 cells at 10 uM after 24 hrs by DCFH-DA probe-based flow cytometric method | 10 | ALA3862022 | cell-based format | Scientific Literature | ||
2. | ALA3863728 | Binding | Inhibition of HDAC in human HeLa cell nuclear extracts at 20 uM preincubated for 5 mins followed by Boc-Lys(Ac)-AMC substrate addition measured after 30 mins by fluorescence assay | Homo sapiens | 10 | ALA3862022 | cell-based format | Scientific Literature | |
3. | ALA3863729 | Functional | Cytotoxicity against human PC3 cells assessed as growth inhibition after 48 hrs by SRB assay | Homo sapiens | 11 | ALA3862022 | cell-based format | Scientific Literature | |
4. | ALA3863730 | Functional | Cytotoxicity against human MCF7 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
5. | ALA3863731 | Functional | Cytotoxicity against human T47D cells assessed as growth inhibition after 24 to 48 hrs by MTT assay | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
6. | ALA3863732 | Functional | Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
7. | ALA3863733 | Toxicity | Cytotoxicity against human IMR90 cells assessed as growth inhibition up to 40 uM after 24 to 48 hrs by MTT assay | Homo sapiens | 1 | ALA3862022 | cell-based format | Scientific Literature | |
8. | ALA3863734 | Functional | Induction of DNA damage in human MDA-MB-231 cells assessed as phosphorylation of gammaH2AX at IC50 to 3 times IC50 after 48 hrs by flow cytometric analysis | Homo sapiens | 1 | ALA3862022 | cell-based format | Scientific Literature | |
9. | ALA3863735 | Binding | Induction of CHK1 phosphorylation in human MDA-MB-231 cells at 10 uM after 6 to 24 hrs by Western blot method | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
10. | ALA3863736 | Binding | Induction of CHK2 phosphorylation in human MDA-MB-231 cells at 10 uM after 6 to 24 hrs by Western blot method | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
11. | ALA3863737 | Binding | Induction of ATM phosphorylation in human MDA-MB-231 cells at 10 uM after 6 to 24 hrs by Western blot method | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
12. | ALA3863738 | Binding | Induction of CHK1/2 phosphorylation in human MDA-MB-231 cells assessed as increase in p53 phosphorylation at 10 uM after 6 to 24 hrs by Western blot method | Homo sapiens | 2 | ALA3862022 | cell-based format | Scientific Literature | |
13. | ALA3863739 | Binding | Induction of CHK1/2 phosphorylation in human MDA-MB-231 cells assessed as increase in p21 protein expression at 10 uM after 6 to 24 hrs by Western blot method | Homo sapiens | 1 | ALA3862022 | cell-based format | Scientific Literature | |
14. | ALA3863740 | Binding | Binding affinity to DNA minor groove in human MDA-MB-231 cells up to 100 uM after 20 mins by Hoechst 33342 staining-based fluorometric method | Homo sapiens | 1 | ALA3862022 | cell-based format | Scientific Literature | |
15. | ALA3863741 | Functional | Induction of apoptosis in human MDA-MB-231 cells assessed as PARP cleavage by flow cytometric method | Homo sapiens | 1 | ALA3862022 | cell-based format | Scientific Literature | |
16. | ALA3863742 | Functional | Cell cycle arrest in human MDA-MB-231 cells assessed as accumulation at S phase at 32 uM after 6 to 12 hrs by propidium iodide staining-based flow cytometric method | Homo sapiens | 1 | ALA3862022 | cell-based format | Scientific Literature |