# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3866555 | B | Inhibition of His-sumo-tagged BALB/c mouse thymus histone methyltransferase G9a (969 to 1263 residues) catalytic domain expressed in Escherichia coli BL21 (DE3) assessed as response unit at 10 to 100 uM by SPR assay | Mus musculus | 6 | ALA3865808 | assay format | Scientific Literature | |
2. | ALA3866556 | B | Inhibition of His-sumo-tagged BALB/c mouse thymus histone methyltransferase G9a (969 to 1263 residues) using biotin labeled peptide substrate at 10 uM after 30 mins in presence of [methyl-3H]-SAM by microbeta scintillation counting relative to control | Mus musculus | 6 | ALA3865808 | single protein format | Scientific Literature | |
3. | ALA3866557 | B | Inhibition of histone methyltransferase G9a (unknown origin) assessed as growth inhibition of cells at 10 uM after 48 hrs by cell based CCK8 assay relative to control | Homo sapiens | 6 | ALA3865808 | cell-based format | Scientific Literature | |
4. | ALA3866558 | B | Inhibition of His-sumo-tagged BALB/c mouse thymus histone methyltransferase G9a (969 to 1263 residues) using biotin labeled peptide substrate after 30 mins in presence of [methyl-3H]-SAM by microbeta scintillation counting | Mus musculus | 1 | ALA3865808 | single protein format | Scientific Literature | |
5. | ALA3866559 | B | Inhibition of His-sumo-tagged BALB/c mouse thymus histone methyltransferase G9a (969 to 1263 residues) catalytic domain expressed in Escherichia coli BL21 (DE3) by SPR assay | Mus musculus | 2 | ALA3865808 | assay format | Scientific Literature | |
6. | ALA3866560 | B | Inhibition of His-sumo-tagged BALB/c mouse thymus histone methyltransferase G9a (969 to 1263 residues) catalytic domain expressed in Escherichia coli BL21 (DE3) using histone H3 (1 to 24 residues) as substrate assessed as reduction in H3K9me2 levels at 10 uM after 1 hr in presence of SAM by MALDI-TOF-mass spectroscopic analysis | Mus musculus | 1 | ALA3865808 | assay format | Scientific Literature | |
7. | ALA3866561 | F | Antiproliferative activity against human K562 cells after 48 hrs by CCK8 assay | Homo sapiens | 2 | ALA3865808 | cell-based format | Scientific Literature | |
8. | ALA3866562 | F | Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay | Homo sapiens | 2 | ALA3865808 | cell-based format | Scientific Literature | |
9. | ALA3866563 | F | Antiproliferative activity against human HepG2 cells after 48 hrs by CCK8 assay | Homo sapiens | 2 | ALA3865808 | cell-based format | Scientific Literature | |
10. | ALA3866564 | F | Antiproliferative activity against human A549 cells after 48 hrs by CCK8 assay | Homo sapiens | 2 | ALA3865808 | cell-based format | Scientific Literature | |
11. | ALA3866565 | B | Inhibition of histone methyltransferase G9a in human K562 cells assessed as reduction in histone H3K9me2 levels at 0.5 to 2 uM after 72 hrs by Western blot analysis | Homo sapiens | 2 | ALA3865808 | cell-based format | Scientific Literature | |
12. | ALA3866566 | F | Inhibition of histone H3K27 trimethylation in human K562 cells at 1 to 2 uM after 72 hrs by Western blot analysis | Homo sapiens | 1 | ALA3865808 | cell-based format | Scientific Literature | |
13. | ALA3866567 | F | Induction of apoptosis in human K562 cells at 2 uM after 24 hrs by Annexin V-PE/7AAD-dual staining based FACS analysis | Homo sapiens | 1 | ALA3865808 | cell-based format | Scientific Literature | |
14. | ALA3866568 | F | Cell cycle arrest in human K562 cells assessed as accumulation at G2/M phase up to 4 uM after 24 hrs by propidium iodide-staining based FACS analysis | Homo sapiens | 1 | ALA3865808 | cell-based format | Scientific Literature |