| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3855157 | B | Inhibition of GST-tagged Caenorhabditis elegans CRN-4 exonuclease activity expressed in Escherichia coli BL21 (DE3) pLysS preincubated for 10 mins followed by [gamma-32P]ATP labelled 11-nucleotide ssRNA substrate addition for 30 mins by PAGE based autoradiography | Caenorhabditis elegans | 9 | assay format | Scientific Literature | ||
| 2. | ALA3855158 | B | Inhibition of N-terminal His-tagged recombinant Escherichia coli K-12 RNase T exonuclease activity expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL preincubated for 10 mins followed by [gamma-32P]ATP labelled 11-nucleotide ssDNA substrate addition for 40 mins by PAGE based autoradiography | Escherichia coli K-12 | 9 | assay format | Scientific Literature | ||
| 3. | ALA3855160 | B | Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssRNA binding to Caenorhabditis elegans GST-tagged CRN-4 expressed in Escherichia coli BL21 (DE3) pLysS at 1 to 10 mM preincubated for 10 mins followed by substrate addition measured after 20 mins by TBE gel electrophoresis mobility shift assay | Caenorhabditis elegans | 1 | assay format | Scientific Literature | ||
| 4. | ALA3855161 | B | Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssDNA binding to recombinant Escherichia coli K-12 N-terminal His-tagged RNase T expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL at 1 mM preincubated for 10 mins followed by substrate addition measured after 20 mins by TBE gel electrophoresis mobility shift assay | Escherichia coli K-12 | 9 | assay format | Scientific Literature | ||
| 5. | ALA3855162 | B | Inhibition of Lassa virus N-terminal His-tagged NP exonuclease domain (342 to 569 residues) expressed in Escherichia coli Tuner (DE3) using FAM-labeled 20-nucleotide ssRNA preincubated for 10 mins followed by substrate addition measured after 60 mins by PAGE based autoradiography | Mammarenavirus lassaense | 9 | assay format | Scientific Literature | ||
| 6. | ALA3855163 | B | Inhibition of Lassa virus N-terminal His-tagged NP exonuclease domain (342 to 569 residues) expressed in Escherichia coli Tuner (DE3) using 3'-overhang 4-nucleotide stem-loop RNA preincubated for 10 mins followed by substrate addition measured after 60 mins by PAGE based autoradiography | Mammarenavirus lassaense | 9 | assay format | Scientific Literature |
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