# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3888596 | B | Caliper Mobility-Shift Assay: To a 96-well plate, 20 μL of 10 μM FL-cGMP as the substrate was added; then 1 μL solution of the compound in DMSO or DMSO solution without compound was added; and then 29 μL of 0.28 ng/μL PDE-11 enzyme buffer (100 mM Hepes pH 7.5, 5 mM MgCl2, 0.002% Brij-35) was added. The maximum final concentration of the compound was 10 μM. The plate was incubated at 30° C. for 1 h, and 20 μL of 70 μM EDTA was then added to stop the reaction. 26 μL of solution of each well was transferred to a 384-well plate. | 15 | single protein format | BindingDB Database |