#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888194	Binding	Nav1.7 In Vitro PX Assay: HEK 293 cells stably transfected with human Nav1.7 were recorded in whole cell voltage clamp mode with the PatchXpress automated electrophysiology system (Molecular Devices, LLC, Sunnyvale, Calif.). Compound effects were measured on a partially inactivated state of the sodium channel. Cells were clamped to a holding potential yielding 20 to 50% inactivation. To elicit sodium current, channels were activated by pulsing to -10 mV for 20 msec. This voltage protocol was repeated at a rate of 0.1 Hz throughout the experiment. A single concentration of test compound was applied to cells for a duration of 3 minutes.		641	ALA3886193	cell-based format	BindingDB Database
2.	ALA3888195	Binding	Nav1.5 In Vitro PX Assay: 293 cells stably transfected with Nav 1.5 were recorded in whole cell voltage clamp mode with the PatchXpress automated electrophysiology system according the manufacturer's specifications (Molecular Devices, LLC, Sunnyvale, Calif.). Cells were held at a holding potential of -50 mV to inactivate sodium channels. To elicit sodium currents the voltage was changed -120 mV to recover a portion of the channels, followed by delivery of test pulses of 20 msec duration to 0 mV, at 0.1 Hz. A single concentration of test compound was applied to cells for a duration of 5 minutes.		14	ALA3886193	cell-based format	BindingDB Database
3.	ALA4321987	Binding	Inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	15	ALA4321785	cell-based format	Scientific Literature
4.	ALA4321991	Binding	Inhibition of human Nav1.1 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	11	ALA4321785	cell-based format	Scientific Literature
5.	ALA4321992	Binding	Inhibition of human Nav1.2 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	12	ALA4321785	cell-based format	Scientific Literature
6.	ALA4321993	Binding	Inhibition of human Nav1.3 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	8	ALA4321785	cell-based format	Scientific Literature
7.	ALA4321994	Binding	Inhibition of human Nav1.4 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	11	ALA4321785	cell-based format	Scientific Literature
8.	ALA4321995	Binding	Inhibition of human Nav1.5 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	14	ALA4321785	cell-based format	Scientific Literature
9.	ALA4321996	Binding	Inhibition of human Nav1.6 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	13	ALA4321785	cell-based format	Scientific Literature
10.	ALA4321998	Binding	Selectivity index, ratio of IC50 for inhibition of human Nav1.1 expressed in HEK293 cells by electrophysiology assay to IC50 for inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	11	ALA4321785	cell-based format	Scientific Literature
11.	ALA4321999	Binding	Selectivity index, ratio of IC50 for inhibition of human Nav1.2 expressed in HEK293 cells by electrophysiology assay to IC50 for inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	12	ALA4321785	cell-based format	Scientific Literature
12.	ALA4322000	Binding	Selectivity index, ratio of IC50 for inhibition of human Nav1.3 expressed in HEK293 cells by electrophysiology assay to IC50 for inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	8	ALA4321785	cell-based format	Scientific Literature
13.	ALA4322001	Binding	Selectivity index, ratio of IC50 for inhibition of human Nav1.4 expressed in HEK293 cells by electrophysiology assay to IC50 for inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	11	ALA4321785	cell-based format	Scientific Literature
14.	ALA4322002	Binding	Selectivity index, ratio of IC50 for inhibition of human Nav1.5 expressed in HEK293 cells by electrophysiology assay to IC50 for inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	14	ALA4321785	cell-based format	Scientific Literature
15.	ALA4322003	Binding	Selectivity index, ratio of IC50 for inhibition of human Nav1.6 expressed in HEK293 cells by electrophysiology assay to IC50 for inhibition of human Nav1.7 expressed in HEK293 cells by electrophysiology assay	Homo sapiens	13	ALA4321785	cell-based format	Scientific Literature
16.	ALA4322005	Binding	Inhibition of mouse Nav1.7 expressed in HEK293 cells by electrophysiology assay	Mus musculus	4	ALA4321785	cell-based format	Scientific Literature
17.	ALA4322006	Binding	Inhibition of rat Nav1.7 expressed in HEK293 cells by electrophysiology assay	Rattus norvegicus	4	ALA4321785	cell-based format	Scientific Literature
18.	ALA4322009	ADME	Unbound fraction in mouse plasma	Mus musculus	5	ALA4321785	cell-free format	Scientific Literature
19.	ALA4322016	ADME	Ratio of unbound plasma concentration in mouse model of histamine-induced pain to IC50 for inhibition of mouse Nav1.7 expressed in HEK293 cells by electrophysiology assay	Mus musculus	1	ALA4321785	cell-based format	Scientific Literature
20.	ALA4322017	ADME	Ratio of unbound plasma concentration in mouse model of UVB-induced pain to IC50 for inhibition of mouse Nav1.7 expressed in HEK293 cells by electrophysiology assay	Mus musculus	1	ALA4321785	cell-based format	Scientific Literature