# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3887619 | B | Enzyme Assay: Briefly, rPRCP was incubated in the presence or absence of various PRCP inhibitors in HEPES-carbonated buffer containing 1 mM Ala-Pro-paranitroaniline (APpNA, a PRCP chromogenic substrate). The final volume was 100 μL and the time of incubation was 60 min. Negative control had only 1 mM APpNA in HEPES-Carbonated buffer. Generation of free p-nitroaniline from APpNA was determined by monitoring changes in absorbance at 405 nm. Assays were done a minimum of 3 times. | Homo sapiens | 30 | single protein format | BindingDB Database |