#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3889212	B	Scintillation Proximity Assay (SPA): The binding of potential ligands to RORγ is measured by competition with [3H] 25-hydroxycholesterol (Perkin Elmer NET674250UC) using a scintillation proximity assay (SPA) binding assay. The ligand binding domain of human RORγ (A262-S507) with an N-terminal His tag is expressed in E. coli and purified using nickel affinity chromatography. 15 ug/well RORγ (A262-S507) is incubated with test compound at varying concentrations in 3-fold serial dilution, with final concentrations ranging from 16.6 uM to 0.28 nM for 10 min at room temperature in PBS buffer (Invitrogen #14190-144) containing 0.5% fatty acid free BSA (Gemini Bio-Products, Cat. #700-107P) and 0.1% Glycerol (Sigma Cat#G5516). 10 nM of [3H] 25-hydroxycholesterol is then added, and the reaction is incubated for 10 min. 10 mg/mL of Copper-His Tag-PVT beads (Perkin Elmer cat #RPNQ0095) are added, and the mixture is incubated for 60 min. The reaction is read on a TopCount Microplate scintillation plate reader (Perkin Elmer). The competition data of the test compound over a range of concentrations was plotted as percentage inhibition of radioligand specifically bound in the absence of test compound (percent of total signal). After correcting for non-specific binding, IC50 values were determined. The IC50 value is defined as the concentration of test compound needed to reduce [3H] 25-hydroxycholesterol specific binding by 50% and is calculated using the four parameter logistic equation to fit the normalized data.		565	ALA3886992	assay format	BindingDB Database
2.	ALA4390455	A	Stability in human liver microsomes assessed as parent compound remaining after 10 mins	Homo sapiens	35	ALA4387773	microsome format	Scientific Literature
3.	ALA4390456	B	Inverse agonist activity at human Gal4-fused RORgammat LBD (267 to 516 residues) expressed in human Jurkat cells measured after 18 hrs by steady-glo luciferase reporter gene assay	Homo sapiens	34	ALA4387773	cell-based format	Scientific Literature
4.	ALA4390457	B	Transactivation of PXR (unknown origin) expressed in human HepG2 cells	Homo sapiens	34	ALA4387773	cell-based format	Scientific Literature
5.	ALA4390458	B	Transactivation of PXR (unknown origin) expressed in human HepG2 cells assessed as maximum receptor activation response relative to control	Homo sapiens	34	ALA4387773	cell-based format	Scientific Literature
6.	ALA4390459	A	Stability in mouse liver microsomes assessed as parent compound remaining after 10 mins	Mus musculus	35	ALA4387773	microsome format	Scientific Literature
7.	ALA4390460	B	Inverse agonist activity at human Gal4-fused RORalpha LBD (272 to 556 residues) expressed in human Jurkat cells measured after 18 hrs by steady-glo luciferase reporter gene assay	Homo sapiens	35	ALA4387773	cell-based format	Scientific Literature
8.	ALA4390461	B	Inverse agonist activity at human Gal4-fused RORbeta LBD (200 to 559 residues) expressed in human Jurkat cells measured after 18 hrs by steady-glo luciferase reporter gene assay	Homo sapiens	35	ALA4387773	cell-based format	Scientific Literature
9.	ALA4390462	B	Transactivation of human GAL4-fused LXRalpha expressed in African green monkey CV1 cells	Homo sapiens	35	ALA4387773	cell-based format	Scientific Literature
10.	ALA4390463	B	Transactivation of human GAL4-fused LXRbeta expressed in African green monkey CV1 cells	Homo sapiens	35	ALA4387773	cell-based format	Scientific Literature