# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3861914 | B | Inhibition of purified human placental microsomal 17beta-HSD2 using [3H]-E2 substrate and NAD+ by HPLC based radioactive displacement assay | Homo sapiens | 6 | ALA3860110 | assay format | Scientific Literature | |
2. | ALA3861917 | B | Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS at 2 uM using [3H]-E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assay | Homo sapiens | 32 | ALA3860110 | assay format | Scientific Literature | |
3. | ALA3861918 | B | Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assay | Homo sapiens | 32 | ALA3860110 | assay format | Scientific Literature | |
4. | ALA3861919 | B | Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs in presence of 0.5% v/v Tween 20 using purified enzyme by fluorimetric assay | Homo sapiens | 31 | ALA3860110 | assay format | Scientific Literature | |
5. | ALA3861920 | B | Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs in absence of 0.5% v/v Tween 20 using purified enzyme by fluorimetric assay | Homo sapiens | 31 | ALA3860110 | assay format | Scientific Literature | |
6. | ALA3861921 | B | Inhibition of purified human placental cytosolic 17beta-HSD1 at 1 uM using [3H]-E1 substrate and NADH by HPLC based radioactive displacement assay | Homo sapiens | 9 | ALA3860110 | assay format | Scientific Literature | |
7. | ALA3861922 | B | Inhibition of purified human placental microsomal 17beta-HSD2 at 1 uM using [3H]-E2 substrate and NAD+ by HPLC based radioactive displacement assay | Homo sapiens | 9 | ALA3860110 | assay format | Scientific Literature | |
8. | ALA3861923 | B | Selectivity index, ratio of Ki for purified human placental microsomal 17beta-HSD2 to KI for N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS | Homo sapiens | 2 | ALA3860110 | assay format | Scientific Literature | |
9. | ALA4155463 | B | Inhibition of human placental cytosolic 17beta-HSD1 using [2,4,6,7-3H]-labeled E1/E1 as substrate and NADH at 1 uM after 10 mins by HPLC based radioflow detection method | Homo sapiens | 14 | ALA4152285 | assay format | Scientific Literature | |
10. | ALA4155464 | P | Solubility of the compound in PBS at 200 uM at pH 7.4 measured after 24 hrs | 15 | ALA4152285 | small-molecule physicochemical format | Scientific Literature | ||
11. | ALA4155465 | B | Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric method | Homo sapiens | 17 | ALA4152285 | assay format | Scientific Literature | |
12. | ALA4155468 | B | Inhibition of human placental cytosolic 17beta-HSD2 using [2,4,6,7-3H]-labeled E2/E2 as substrate and NADH at 1 uM after 10 mins by HPLC based radioflow detection method | Homo sapiens | 14 | ALA4152285 | assay format | Scientific Literature | |
13. | ALA4155469 | B | Inhibition of human full length N-terminal GST-tagged 17beta-HSD10 expressed in Escherichia coli BL21 DE3 Codon plus using [6,7-3H]-labeled E2/E2 as substrate and NADH at 1 uM after 30 mins by HPLC based radioflow detection method | Homo sapiens | 14 | ALA4152285 | assay format | Scientific Literature | |
14. | ALA4155470 | A | Agonist activity at ERalpha (unknown origin) expressed in human U2OS cells after 24 hrs by luciferase reporter gene assay relative to E2 | Homo sapiens | 1 | ALA4152285 | cell-based format | Scientific Literature | |
15. | ALA4155472 | A | Cytotoxicity against HEK293 cells assessed as decrease in cell viability after 72 hrs by MTT assay | Homo sapiens | 5 | ALA4152285 | cell-based format | Scientific Literature | |
16. | ALA4155473 | A | Cytotoxicity against HEK293 cells assessed as cell survival at 20 uM after 72 hrs by MTT assay relative to control | Homo sapiens | 7 | ALA4152285 | cell-based format | Scientific Literature |