# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3881393 | B | Inhibition of rat NHE3-mediated sodium-dependant hydrogen ion antiport in Opossum kidney cells preincubated in NH4Cl-HEPES buffer containing BCECF-AM dye followed by washout with ammonium free HEPES and subsequent addition of compound in presence of NHE1 antagonist EIPA by fluorescence assay | Rattus norvegicus | 179 | cell-based format | Patent Bioactivity Data | ||
2. | ALA3881394 | B | Inhibition of human NHE3-mediated sodium-dependant hydrogen ion antiport in Opossum kidney cells preincubated in NH4Cl-HEPES buffer containing BCECF-AM dye followed by washout with ammonium free HEPES and subsequent addition of compound in presence of NHE1 antagonist EIPA by fluorescence assay | Homo sapiens | 162 | cell-based format | Patent Bioactivity Data | ||
3. | ALA3881396 | F | Effect on sodium level in urine in Sprague-Dawley rat at 1 mg/kg administered via oral gavage measured after 16 hrs by gravimetric analysis relative to vehicle-treated control | Rattus norvegicus | 28 | organism-based format | Patent Bioactivity Data | ||
4. | ALA3881400 | F | Effect on phosphate level in urine in Sprague-Dawley rat at 1 mg/kg administered via oral gavage measured after 16 hrs by gravimetric analysis relative to vehicle-treated control | Rattus norvegicus | 26 | organism-based format | Patent Bioactivity Data | ||
5. | ALA3881403 | B | Inhibition of rat NHE3-mediated sodium-dependant hydrogen ion antiport in Opossum kidney cells preincubated for 60 mins in NaCl-HEPES buffer followed by compound washout with NH4Cl-HEPES buffer containing BCECF-AM dye and subsequent washout with Na+ free HEPES buffer by fluorescence analysis | Rattus norvegicus | 5 | cell-based format | Patent Bioactivity Data | ||
6. | ALA3881404 | B | Inhibition of human NHE3-mediated sodium-dependant hydrogen ion antiport in Opossum kidney cells preincubated for 60 mins in NaCl-HEPES buffer followed by compound washout with NH4Cl-HEPES buffer containing BCECF-AM dye and subsequent washout with Na+ free HEPES buffer by fluorescence analysis | Homo sapiens | 5 | cell-based format | Patent Bioactivity Data | ||
7. | ALA3881405 | B | Inhibition of human Nap2B expressed in HEK293 cells assessed as inhibition of Pi uptake measured after 23 mins in presence of [33P]orthophosphoric acid by TopCount scintillation counting method | Homo sapiens | 5 | cell-based format | Patent Bioactivity Data | ||
8. | ALA5149726 | B | Inhibition of human NHE3 expressed in opossum kidney cells assessed as reduction in Na-HEPES buffer-mediated pH recovery in presence of NEH1 inhibitor ethyl isopropyl amiloride by BCECF-AM dye based fluorescence assay | Homo sapiens | 46 | cell-based format | Scientific Literature | ||
9. | ALA5149727 | B | Inhibition of rat NHE3 expressed in opossum kidney cells assessed as reduction in Na-HEPES buffer-mediated pH recovery in presence of NEH1 inhibitor ethyl isopropyl amiloride by BCECF-AM dye based fluorescence assay | Rattus norvegicus | 43 | cell-based format | Scientific Literature |