#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888488	B	In Vitro Assay: HEK 293 cells stably transfected with either Nav 1.7 or with Nav 1.5 were recorded in population patch-clamp mode with the IonWorks® Quattro automated electrophysiology system in accordance with the manufacturer's specifications (Molecular Devices, LLC, Sunnyvale, Calif.). Sodium channel currents were measured in response to a train of depolarizations that induced successively greater inactivation. Cells were held at −110 mV for three seconds (Nav 1.7) or half a second (Nav 1.5) from a holding voltage of −15 mV, then put through a series of 26 pulses of 150 msec duration to −20 mV at a frequency of 5 Hz. Cells were then left unclamped for a period of 3 to 8 minutes while a single concentration of test compound was added. Cells were then reclamped and put through the same voltage protocol. Current at the end of the 26th pulse to −20 mV was subtracted from the peak current evoked by the 26th pulse to −20 mV to correct for leak current.		218	ALA3886728	cell-based format	BindingDB Database
2.	ALA3888489	B	In Vitro PX Assay: HEK 293 cells stably transfected with human Nav1.7 were recorded in whole cell voltage clamp mode with the PatchXpress automated electrophysiology system (Molecular Devices, LLC, Sunnyvale, Calif.). Compound effects were measured on a partially inactivated state of the sodium channel. Cells were clamped to a holding potential yielding 20 to 50% inactivation. To elicit sodium current, channels were activated by pulsing to −10 mV for 20 msec. This voltage protocol was repeated at a rate of 0.1 Hz throughout the experiment. A single concentration of test compound was applied to cells for a duration of 3 minutes.		234	ALA3886728	cell-based format	BindingDB Database
3.	ALA3888490	B	In Vitro Assay: HEK 293 cells stably transfected with either Nav 1.7 or with Nav 1.5 were recorded in population patch-clamp mode with the IonWorks® Quattro automated electrophysiology system in accordance with the manufacturer's specifications (Molecular Devices, LLC, Sunnyvale, Calif.). Sodium channel currents were measured in response to a train of depolarizations that induced successively greater inactivation. Cells were held at −110 mV for three seconds (Nav 1.7) or half a second (Nav 1.5) from a holding voltage of −15 mV, then put through a series of 26 pulses of 150 msec duration to −20 mV at a frequency of 5 Hz. Cells were then left unclamped for a period of 3 to 8 minutes while a single concentration of test compound was added. Cells were then reclamped and put through the same voltage protocol. Current at the end of the 26th pulse to −20 mV was subtracted from the peak current evoked by the 26th pulse to −20 mV to correct for leak current.		220	ALA3886728	cell-based format	BindingDB Database
4.	ALA4055938	B	Inhibition of human NaV1.7 expressed in HEK293 cells at holding potential of -125 mV with test potentials to -10 mV by whole cell voltage clamp method	Homo sapiens	17	ALA4052683	cell-based format	Scientific Literature
5.	ALA4055939	B	Inhibition of rat NaV1.7 by cell based whole cell voltage clamp method	Rattus norvegicus	13	ALA4052683	cell-based format	Scientific Literature
6.	ALA4055940	B	Inhibition of human NaV1.5 expressed in HEK295 cells at holding potential of -125 mV with test potentials to -10 mV by whole cell voltage clamp method	Homo sapiens	13	ALA4052683	cell-based format	Scientific Literature
7.	ALA4055942	A	Unbound clearance in rat at 0.5 mg/kg, iv by LC-MS/MS method	Rattus norvegicus	13	ALA4052683	organism-based format	Scientific Literature
8.	ALA4055943	A	Apparent permeability in pig LLC-PK1 cells expressing MDR1 at 5 uM	Sus scrofa	16	ALA4052683	cell-based format	Scientific Literature
9.	ALA4055944	A	Clearance in rat at 0.5 mg/kg, iv by LC-MS/MS method	Rattus norvegicus	13	ALA4052683	organism-based format	Scientific Literature
10.	ALA4055945	A	Clearance in pooled rat liver microsomes at 1 uM in presence of NADPH by LC-MS/MS method	Rattus norvegicus	14	ALA4052683	assay format	Scientific Literature
11.	ALA4055946	A	Clearance in pooled human liver microsomes at 1 uM in presence of NADPH by LC-MS/MS method	Homo sapiens	14	ALA4052683	assay format	Scientific Literature
12.	ALA4055948	A	Unbound fraction in rat plasma at 5 uM by ultracentrifugation and LC-MS/MS method	Rattus norvegicus	1	ALA4052683	cell-free format	Scientific Literature
13.	ALA4055949	B	Inhibition of NaV1.8 in rat DRG neurons assessed as reduction in tetrodotoxin-sensitive sodium channel currents at holding potential of -140 mV by manual patch clamp electrophysiology assay	Rattus norvegicus	1	ALA4052683	assay format	Scientific Literature
14.	ALA4055950	B	Inhibition of NaV1.8 in C57BL/6 mouse DRG neurons assessed as reduction in tetrodotoxin-sensitive sodium channel currents at holding potential of -140 mV by manual patch clamp electrophysiology assay	Mus musculus	1	ALA4052683	assay format	Scientific Literature
15.	ALA4055951	A	Unbound fraction in mouse plasma at 5 uM by ultracentrifugation and LC-MS/MS method	Mus musculus	1	ALA4052683	cell-free format	Scientific Literature
16.	ALA4055953	B	Inhibition of cynomolgus monkey NaV1.7 by whole cell voltage clamp method	Macaca fascicularis	4	ALA4052683	assay format	Scientific Literature
17.	ALA4055954	B	Inhibition of mouse NaV1.7 by whole cell voltage clamp method	Mus musculus	4	ALA4052683	single protein format	Scientific Literature
18.	ALA4055955	A	Protein binding in rat plasma at 5 uM by ultracentrifugation and LC-MS/MS method	Rattus norvegicus	1	ALA4052683	cell-free format	Scientific Literature
19.	ALA4055956	A	Protein binding in mouse plasma at 5 uM by ultracentrifugation and LC-MS/MS method	Mus musculus	1	ALA4052683	cell-free format	Scientific Literature
20.	ALA4055957	A	AUC (infinity) in rat at 0.5 mg/kg, iv by LC-MS/MS method	Rattus norvegicus	1	ALA4052683	organism-based format	Scientific Literature