#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3867372	B	Inhibition of Toxoplasma gondii TS-DHFR expressed in Escherichia coli BL21 preincubated for 15 mins followed by addition of DHF as substrate and NADPH measured after 60 mins by resazurin/diaphorase coupled assay	Toxoplasma gondii	24	ALA3865850	cell-free format	Scientific Literature
2.	ALA3867373	A	Inhibition of recombinant human DHFR expressed in Escherichia coli preincubated for 15 mins followed by addition of DHF as substrate and NADPH measured after 60 mins by resazurin/diaphorase coupled assay	Homo sapiens	24	ALA3865850	assay format	Scientific Literature
3.	ALA3867374	B	Selectivity ratio of IC50 for human DHFR to IC50 for Toxoplasma gondii DHFR		24	ALA3865850	assay format	Scientific Literature
4.	ALA4423789	B	Inhibition of Toxoplasma gondii DHFR-TS expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and NADPH addition and measured after 60 mins by resazurin dye based diaphorase-coupled assay	Toxoplasma gondii	31	ALA4422638	cell-based format	Scientific Literature
5.	ALA4423790	A	Inhibition of human DHFR expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and NADPH addition and measured after 60 mins by resazurin dye based diaphorase-coupled assay	Homo sapiens	31	ALA4422638	cell-based format	Scientific Literature
6.	ALA4423791	T	Selectivity ratio of IC50 for human DHFR to IC50 for Toxoplasma gondii DHFR-TS		29	ALA4422638	assay format	Scientific Literature
7.	ALA4423792	P	Kinetic solubility of the compound at pH 7 after 24 hrs by shake flask method		12	ALA4422638	small-molecule physicochemical format	Scientific Literature
8.	ALA4423793	A	Intrinsic clearance in human liver microsomes at 10 uM preincubated for 10 mins followed by NADPH addition and measured up to 60 mins by LC/MS/MS analysis	Homo sapiens	13	ALA4422638	microsome format	Scientific Literature
9.	ALA4423794	A	Apparent permeability from apical to basolateral side in MDCK-MDR1 cells at 2 uM after 15 mins by LC/MS/MS analysis	Canis lupus familiaris	10	ALA4422638	cell-based format	Scientific Literature
10.	ALA4423795	A	Efflux ratio of apparent permeability in MDCK-MDR1 cells at 2 uM after 15 mins by LC/MS/MS analysis	Canis lupus familiaris	10	ALA4422638	cell-based format	Scientific Literature