#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888196	B	HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	Homo sapiens	50		single protein format	BindingDB Database
2.	ALA3888198	B	HDAC Activity Assay: HDAC assay is performed using fluorescently-labeled acetylated substrate, which comprises an acetylated lysine side chain. After incubation with HDAC, deacetylation of the substrate sensitizes the substrate such that, in a second step, treatment with the detection enzyme produces a fluorophore. HDACs 1 and 6 were expressed as full length fusion proteins. Purified proteins were incubated with 50 μM fluorescently-labeled acetylated peptide substrate and test compound for 2 hours at RT in HDAC assay buffer containing 50 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% DMSO, and 1% BSA. Reactions were terminated by the addition of the Developer after 2 hours, and the development of fluorescence signal, which was relative to the amount of deacetylated peptide, was monitored by time-course measurement of EnVision (PerkinElmer). The HDAC activity was estimated from the slope of time-course measurement of the fluorescence intensity.	Homo sapiens	50		single protein format	BindingDB Database
3.	ALA4416947	B	Inhibition of recombinant full length human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 insect cells pretreated with compound followed by Fluor de Lys deacetylase substrate addition by fluorescence method	Homo sapiens	64		cell-based format	Scientific Literature
4.	ALA4416950	U	Selectivity ratio of IC50 for C-terminal His-tagged human HDAC3 (1 to 428 residues)/N-terminal GST tagged human NCOR2 (395 to 489) expressed in baculovirus infected sf9 insect cells to human N-terminal GST-tagged HDAC6 expressed in baculovirus infected sf9 insect cells	Homo sapiens	64		cell-based format	Scientific Literature
5.	ALA4615734	B	Inhibition of HDAC6 in mouse N2A cells assessed as increase in alpha tubulin acetylation	Mus musculus	3		cell-based format	Scientific Literature
6.	ALA4615735	B	Inhibition of HDAC1 in mouse N2A cells	Mus musculus	3		cell-based format	Scientific Literature
7.	ALA4615736	B	Inhibition of HDAC6 in mouse N2A cells	Mus musculus	3		cell-based format	Scientific Literature
8.	ALA4615737	B	Selectivity index, ratio of IC50 for HDAC1 in mouse N2A cells to IC50 for HDAC6 in mouse N2A cells	Mus musculus	3		cell-based format	Scientific Literature
9.	ALA4615738	B	Ratio of EC50 for inhibition of HDAC6 in mouse N2A cells assessed as alpha tubulin acetylation to IC50 for HDAC6 in mouse N2A cells	Mus musculus	3		cell-based format	Scientific Literature
10.	ALA4615739	A	Efflux ratio of permeability in MDCK-MDR1 cells	Canis lupus familiaris	3		cell-based format	Scientific Literature
11.	ALA4615740	A	Drug concentration in CD-1 mouse brain at 3 mg/kg, iv measured at 8 mins	Mus musculus	3		organism-based format	Scientific Literature
12.	ALA4615741	A	Drug concentration in CD-1 mouse brain at 3 mg/kg, iv measured at 60 mins	Mus musculus	3		organism-based format	Scientific Literature
13.	ALA4615742	A	Drug concentration in CD-1 mouse plasma at 3 mg/kg, iv measured at 8 mins	Mus musculus	3		organism-based format	Scientific Literature
14.	ALA4615743	A	Drug concentration in CD-1 mouse plasma at 3 mg/kg, iv measured at 60 mins	Mus musculus	3		organism-based format	Scientific Literature
15.	ALA4615744	A	Ratio of drug level in brain to plasma in CD-1 mouse at 3 mg/kg, iv measured after 8 mins	Mus musculus	3		organism-based format	Scientific Literature
16.	ALA4615745	A	Ratio of drug level in brain to plasma in CD-1 mouse at 3 mg/kg, iv measured after 60 mins	Mus musculus	3		organism-based format	Scientific Literature
17.	ALA4615748	F	In vivo inhibition of HDAC6 in C57BL/6 mouse cortex assessed as increase in alpha tubulin acetylation at 20 mg/kg, ip administered bid with interval of 4 hrs and measured after 1.5 hrs of last dosage by Western blot analysis	Mus musculus	3		organism-based format	Scientific Literature