#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888056	B	Biochemical Kinase Assay: Liquid handling and incubation steps were done on an Innovadyne Nanodrop Express equipped with a robotic arm (Thermo CatX, Caliper Twister II) and an incubator (Liconic STX40, Thermo Cytomat 2C450). The assay plates were prepared by addition of 50 nl per well of compound solution in 90% DMSO. The kinase reactions were started by stepwise addition of 4.5 ul per well of peptide/ATP-solution (50 mM HEPES, pH 7.5, 1 mM DTT, 0.02% Tween20, 0.02% BSA, 0.6% DMSO, 10 mM beta-glycerophosphate, and 10 uM sodium orthovanadate, 16 mM MgCl2, 1122 uM ATP, 4 uM peptide (5-Fluo-Ahx-KKKKEEIYFFFG-NH2, Biosyntan GmbH) and 4.5 ul per well of enzyme solution (50 mM HEPES, pH 7.5, 1 mM DTT, 0.02% Tween20, 0.02% BSA, 0.6% DMSO, 10 mM beta-glycerophosphate, and 10 uM sodium orthovanadate, 16 mM MgCl2, 6 nM FGFR4 (GST-FGFR4(388-802), produced in-house by expression in insect cells and affinity chromatography). Kinase reactions were incubated at 30° C. for 60 minutes and subsequently terminated by addition of 16 ul per well of stop solution (100 mM HEPES pH 7.5, 5% DMSO, 0.1% Caliper coating reagent, 10 mM EDTA, and 0.015% Brij35).		143		cell-based format	BindingDB Database
2.	ALA4723216	A	Plasma protein binding in CD1 mouse at 5 uM measured after 4 hrs by LC-MS/MS based rapid equilibrium dialysis method	Mus musculus	27		cell-free format	Scientific Literature
3.	ALA4723280	A	Permeability of the compound in human Caco-2 cells assessed as compound recovery at 10 uM measured after 2 hrs by LC/MS/MS analysis	Homo sapiens	40		cell-based format	Scientific Literature
4.	ALA4723285	A	Drug metabolism in rat liver microsome assessed as reduction of aldehyde group at 10 mM measured after 15 mins in presence of NADPH by LC-MS analysis	Rattus norvegicus	40		microsome format	Scientific Literature
5.	ALA4723286	A	Drug metabolism in human liver microsome assessed as reduction of aldehyde group at 10 mM measured after 15 mins in presence of NADPH by LC-MS analysis	Homo sapiens	40		microsome format	Scientific Literature
6.	ALA4723292	A	Hepatic extraction ratio in rat liver microsomes preincubated for 3 mins followed by NADPH addition and measured after 60 mins	Rattus norvegicus	80		microsome format	Scientific Literature
7.	ALA4723294	B	Inhibition of FGFR4 in mouse BAF3 cells assessed as reduction in cell viability incubated for 2 days by cell proliferation assay	Mus musculus	45		cell-based format	Scientific Literature
8.	ALA4723295	B	Inhibition of FGFR4 in mouse BAF3 cells assessed as decrease in FGFR4 phosphorylation incubated for 40 mins	Mus musculus	76		cell-based format	Scientific Literature
9.	ALA4723314	B	Inhibition of recombinant non-phosphorylated FGFR4 kinase domain (442 to 753) (unknown origin) expressed in Sf9 insect cells using 5-Fluo-Ahx-KKKKEEIYFFFG-NH2 peptide as substrate in presence of ATP measured after 60 mins by caliper microfluidic mobility shift assay	Homo sapiens	82		cell-based format	Scientific Literature