#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888345	B	Kinase Activity Assay: The kinase reaction buffer consists of 50 mM Tris pH 8.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA, and 2 mM DTT. In the assay, 1 nM LRRK2 WT or 250 pM LRRK2 G2019S kinase is incubated with the test compound (typically at 0 to 30 μM) for 30 minutes before the kinase reaction is initiated by addition of 1.3 mM ATP and 0.4 μM fluorescein-LRRKtide. The reaction mixture (20 μl total volume) is incubated for 2 hours at 30 °C. before the reaction is terminated by addition of 10 mM EDTA and 1 nM terbium-labelled anti-phospho-LRRKtide antibody (final volume 20 μl). The mixture is further incubated for 30 minutes at RT. TR-FRET is measured by excitation of the terbium-donor with 340 nm light and subsequent (delay time 100 μs) measurement of terbium and fluorescein emission at 495 nm and 520 nm, respectively, over a time window of 1000 μs. The measurement is repeated 10 times for fluorescein and 10 times for terbium emission with a 2000 μs time window between repeats. TR-FRET measurements are performed on a Biomek Synergy plate. The TR-FRET signal is calculated as the emission-ratio at 520 nm over 495 nm.		30	ALA3886669	single protein format	BindingDB Database
2.	ALA3888346	B	Kinase Activity Assay: The kinase reaction buffer consists of 50 mM Tris pH 8.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA, and 2 mM DTT. In the assay, 1 nM LRRK2 WT or 250 pM LRRK2 G2019S kinase is incubated with the test compound (typically at 0 to 30 μM) for 30 minutes before the kinase reaction is initiated by addition of 1.3 mM ATP and 0.4 μM fluorescein-LRRKtide. The reaction mixture (20 μl total volume) is incubated for 2 hours at 30 °C. before the reaction is terminated by addition of 10 mM EDTA and 1 nM terbium-labelled anti-phospho-LRRKtide antibody (final volume 20 μl). The mixture is further incubated for 30 minutes at RT. TR-FRET is measured by excitation of the terbium-donor with 340 nm light and subsequent (delay time 100 μs) measurement of terbium and fluorescein emission at 495 nm and 520 nm, respectively, over a time window of 1000 μs. The measurement is repeated 10 times for fluorescein and 10 times for terbium emission with a 2000 μs time window between repeats. TR-FRET measurements are performed on a Biomek Synergy plate. The TR-FRET signal is calculated as the emission-ratio at 520 nm over 495 nm.		29	ALA3886669	single protein format	BindingDB Database
3.	ALA4045603	B	Inhibition of recombinant human LRRK2 (970 to 2527 residues) G2019S mutant expressed in baculovirus preincubated for 30 mins followed by fluorescein-LRRKtide substrate addition after 2 hrs by TR-FRET assay	Homo sapiens	21	ALA4043168	assay format	Scientific Literature
4.	ALA4045604	B	Inhibition of recombinant human wild type GST-tagged LRRK2 (970 to 2527 residues) preincubated for 30 mins followed by fluorescein-LRRKtide substrate addition after 2 hrs by TR-FRET assay	Homo sapiens	15	ALA4043168	single protein format	Scientific Literature
5.	ALA4045611	A	Intrinsic clearance in human liver microsomes	Homo sapiens	2	ALA4043168	assay format	Scientific Literature
6.	ALA4045612	A	Intrinsic clearance in rat liver microsomes	Rattus norvegicus	2	ALA4043168	assay format	Scientific Literature
7.	ALA4045615	P	Aqueous solubility of the compound in pH 7.4		2	ALA4043168	small-molecule physicochemical format	Scientific Literature
8.	ALA4045616	A	Apparent permeability in MDCK cells harboring MDR1	Canis lupus familiaris	1	ALA4043168	cell-based format	Scientific Literature
9.	ALA4045617	A	Efflux ratio of apparent permeability from basolateral to apical over apical to basolateral side in MDCK cells harboring MDR1	Canis lupus familiaris	1	ALA4043168	cell-based format	Scientific Literature
10.	ALA4045618	A	Ratio of drug level in mouse brain to plasma at 10 mg/kg, po	Mus musculus	1	ALA4043168	organism-based format	Scientific Literature
11.	ALA4045619	A	Unbound fraction in mouse brain at 10 mg/kg, po	Mus musculus	1	ALA4043168	organism-based format	Scientific Literature
12.	ALA4045620	B	Inhibition wild type LRRK2 (unknown origin) Ser935 phosphorylation in HEK293 cells	Homo sapiens	1	ALA4043168	cell-based format	Scientific Literature
13.	ALA4045621	B	Inhibition LRRK2 G2019S mutant (unknown origin) Ser935 phosphorylation in HEK293 cells	Homo sapiens	1	ALA4043168	cell-based format	Scientific Literature
14.	ALA4045622	A	Ratio of unbound drug level in mouse brain to plasma at 10 mg/kg, po	Mus musculus	1	ALA4043168	organism-based format	Scientific Literature
15.	ALA4045623	B	Inhibition of AMPKa1 ATP loop (IGHYILGDTLGVGTFGKVK) in human PBMC at 1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
16.	ALA4045624	B	Inhibition of MLKL conserved Lys1 (APVAIKVFK) in human PBMC at 1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
17.	ALA4045625	B	Inhibition of SRC ATP binding site (QGAKFPIKWTAPEAALYGR) in human PBMC at 0.1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
18.	ALA4045626	B	Inhibition of GPRK6 conserved Lys2 (DLKPENILLDDHGHIR) in human PBMC at 0.1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
19.	ALA4045627	B	Inhibition of MLKL conserved Lys1 (APVAIKVFK) in human PBMC at 0.1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature
20.	ALA4045628	B	Inhibition of ABL labeling at activation Loop (LMTGDTYTAHAGAKFPIK) in human PBMC at 1 uM	Homo sapiens	1	ALA4043168	single protein format	Scientific Literature