#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888329	B	In Vitro hLTC4 Synthase Enzyme Assay (Test B): In the assay, LTC4 synthase catalyses the reaction where the substrate LTA4 methyl ester is converted to LTC4 methyl ester. In order to obtain IC50-values for the compounds, the following procedure was used: 10 mL of 0.6 nM of human recombinant purified LTC4 synthase expressed from Pichia Pastoris in buffer 50 mM tris (pH 7.5), 0.05% BSA 0.03% DD M 100 mM NaCl was preincubated for 30 min with 10 mL of substrate mix containing 6 mM LTA4-methyl ester substrate and 1 mM glutathione in 50 mM Tris pH7.5 0.05% BSA 0.03% DDM 100 mM NaCl together with 0.1 μL of compound of interest in DMSO at ten different concentrations, typically in the range 10^−9,5-10^−5 M as well as with DMSO only. The enzymatic reaction is initiated by addition of 10 μL LTC4S enzyme solution to 10 μL substrate solution to the assay plate containing 0.5 mL of compound in DMSO or 0.5 mL DMSO control. The reaction is stopped after 30 min by addition of 40 μL 75% (w/v) acetonitrile in H2O.	Homo sapiens	4	ALA3886663	single protein format	BindingDB Database
2.	ALA4378183	B	Inhibition of human N-terminal His6-tagged LTC4S expressed in Pichia pastoris X33 using LTA4 methyl ester and glutathione as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by LC-MS/MS analysis	Homo sapiens	34	ALA4376816	assay format	Scientific Literature
3.	ALA4378184	B	Inhibition of LTC4S in zymosan-stimulated human PBMC assessed as inhibition of LTC4 production preincubated for 45 mins followed by zymosan stimulation and measured after 20 mins by LC-MS/MS analysis	Homo sapiens	32	ALA4376816	single protein format	Scientific Literature
4.	ALA4378185	P	Octanol/Sodium phosphate buffer partition coefficient, log D of compound at pH 7.4 at 1 mg incubated for overnight under shaking condition by LC-MS/MS analysis		31	ALA4376816	small-molecule physicochemical format	Scientific Literature
5.	ALA4378186	A	Intrinsic clearance in human hepatocytes at 1 uM assessed as per million cells after 2 to 60 mins by LC-MS/MS analysis	Homo sapiens	31	ALA4376816	cell-based format	Scientific Literature