| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3873321 | B | Inhibition of human recombinant 5-LOX pseudoperoxidase activity expressed in Escherichia coli BL21 assessed as 13(S)-HPODE consumption by measuring decrease in absorbance at 10 uM | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 2. | ALA3873322 | B | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as decrease in 5-HPETE production at 50 uM preincubated with enzyme followed by arachidonic acid substrate addition by UV-vis spectrophotometry | Homo sapiens | 16 | assay format | Scientific Literature | ||
| 3. | ALA3873323 | B | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as decrease in 5-HPETE production preincubated with enzyme followed by arachidonic acid substrate addition by UV-vis spectrophotometry | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 4. | ALA3873324 | B | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as enzyme Vmax at 10 uM preincubated with enzyme followed by arachidonic acid substrate addition by Lineweaver-Burk plot method (Rvb = 0.83 +/- 0.06 nmol/min) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA3873325 | B | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as enzyme Vmax at 50 uM preincubated with enzyme followed by arachidonic acid substrate addition by Lineweaver-Burk plot method (Rvb = 0.83 +/- 0.06 nmol/min) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA3873326 | B | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as Km for substrate at 10 uM preincubated with enzyme followed by arachidonic acid substrate addition by Lineweaver-Burk plot method (Rvb = 2.2 uM) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 7. | ALA3873327 | B | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as Km for substrate at 50 uM preincubated with enzyme followed by arachidonic acid substrate addition by Lineweaver-Burk plot method (Rvb = 2.2 uM) | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA3873332 | B | Inhibition of mPGES1 in human HeLa cells assessed as reduction in TNF-alpha induced PGE2 production at 50 uM preincubated for 2 hrs followed by TNF-alpha addition for 24 hrs by ELISA | Homo sapiens | 15 | cell-based format | Scientific Literature | ||
| 9. | ALA3873333 | F | Antioxidant activity assessed as DPPH radical scavenging activity at 50 uM after 30 mins | 16 | assay format | Scientific Literature | |||
| 10. | ALA3873336 | B | Inhibition of COX-2/mPGES1 in human HeLa cells assessed as reduction in TNF-alpha induced PGE2 production at 50 uM after 15 mins followed by ELISA | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA3873337 | B | Inhibition of COX-2/mPGES1 in human HeLa cells assessed as reduction in TNF-alpha induced PGE2 production at 50 uM preincubated for 15 mins followed by arachidonic acid addition for 15 mins by ELISA | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA3873340 | F | Inhibition of COX-2 mRNA expression in human HeLa cells at 50 uM by RT-PCR method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 13. | ALA3873341 | F | Increase in IFN-gamma mRNA expression in human HeLa cells at 50 uM by RT-PCR method relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 14. | ALA3873342 | F | Inhibition of mPGES1 mRNA expression in human HeLa cells at 50 uM by RT-PCR method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 15. | ALA3873343 | F | Inhibition of COX-1 mRNA expression in human HeLa cells at 50 uM by RT-PCR method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 16. | ALA3873347 | F | Increase in IL-6 mRNA expression in human HeLa cells at 50 uM by RT-PCR method relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 17. | ALA3873348 | F | Increase in TGF-beta3 mRNA expression in human HeLa cells at 50 uM by RT-PCR method relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 18. | ALA3873349 | F | Increase in NFkappaB mRNA expression in human HeLa cells at 50 uM by RT-PCR method relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 19. | ALA3873350 | T | Cytotoxicity against human HeLa cells assessed as cell proliferation at 50 uM after 24 hrs by MTT assay | Homo sapiens | 16 | cell-based format | Scientific Literature |
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