#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888033	B	FLIPR Assay: The bioactivity of compounds is tested in a fluorometric imaging plate reader (FLIPR: Molecular Devices) using engineered CHO-K1 cells expressing the human CXCR3A coupled to a G protein (Galpha(16)). Cells are plated the day prior to bioassay in F12 medium supplemented with 10% FBS and G418 and hygromycin antibiotics to maintain recombinant selection. At the day of bioassay, cells are washed and dye loaded for one hour with Fluo-4-AM (Invitrogen) in Hanks Balanced Salt Solution (Invitrogen), buffered with 20 mM Hepes at pH 7.4 and sodium bicarbonate (0.015%), containing 5 mM probenecid. This buffer, but lacking the dye and containing probenecid at a concentration of 2.5 nM, is also is used for washing steps (wash buffer); or lacking both dye and probenecid but supplemented with 0.1% BSA for compound dilution steps (dilution buffer). Cells are washed free of excess dye and 60 microliter of wash buffer is added. Stock solutions of test compounds are made up at a concentration of 10 mM in DMSO, and serially diluted in dilution buffer to concentrations required for inhibition dose response curves. After a 10 minute incubation period at 37° C., 10 microliters of each compound dilution are transferred from a compound plate to the plate containing the recombinant cells in the FLIPR instrument according to the manufacturer's instructions. Following basal readings, 10 microliter CXCL10 agonist at a concentration of 20 nM (from Peprotech) is added, again using the FLIPR instrument. Changes in fluorescence are monitored before and after addition of the test compounds.		243	ALA3886546	cell-based format	BindingDB Database
2.	ALA5130041	F	Antagonist activity at human recombinant CXCR3 expressed in CHO-K1 cells measured after 10 mins in presence of CXCL10 by FLIPR analysis	Homo sapiens	39	ALA5126609	cell-based format	Scientific Literature
3.	ALA5130042	F	Antagonist activity at human recombinant CXCR3 in human venous blood assessed as receptor internalization measured for 30 mins in presence of CXCL10 by flow cytometric analysis	Homo sapiens	39	ALA5126609	tissue-based format	Scientific Literature
4.	ALA5130043	T	Inhibition of hERG expressed in CHO cells at 3 uM at -80 mV holding potential assessed as current block incubated for 3 mins by Qpatch clamp method relative to control	Homo sapiens	39	ALA5126609	cell-based format	Scientific Literature
5.	ALA5130048	P	Lipophilicity, log D of the compound at pH 7.4		13	ALA5126609	small-molecule physicochemical format	Scientific Literature
6.	ALA5130049	P	Solubility of the compound in FaSSIF		6	ALA5126609	small-molecule physicochemical format	Scientific Literature
7.	ALA5130050	P	Solubility of the compound in FeSSIF		13	ALA5126609	small-molecule physicochemical format	Scientific Literature
8.	ALA5130051	A	Intrinsic clearance in human liver microsomes at 1 uM measured up to 15 mins in presence of NADPH by LC-MS/MS analysis	Homo sapiens	19	ALA5126609	microsome format	Scientific Literature
9.	ALA5130053	A	Intrinsic clearance in mouse liver microsomes at 1 uM measured up to 15 mins in presence of NADPH by LC-MS/MS analysis	Mus musculus	10	ALA5126609	microsome format	Scientific Literature
10.	ALA5130054	A	Unbound fraction in mouse plasma by equilibrium based LC-MS/MS analysis	Mus musculus	7	ALA5126609	cell-free format	Scientific Literature
11.	ALA5130055	A	Intrinsic clearance in rat liver microsomes at 1 uM measured up to 15 mins in presence of NADPH by LC-MS/MS analysis	Rattus norvegicus	7	ALA5126609	microsome format	Scientific Literature
12.	ALA5130056	A	Unbound fraction in rat plasma by equilibrium based LC-MS/MS analysis	Rattus norvegicus	5	ALA5126609	cell-free format	Scientific Literature
13.	ALA5130057	A	Cmax in Balb/c mouse at 10 mg/kg, po	Mus musculus	10	ALA5126609	organism-based format	Scientific Literature
14.	ALA5130058	A	Tmax in Balb/c mouse at 10 mg/kg, po	Mus musculus	10	ALA5126609	organism-based format	Scientific Literature
15.	ALA5130059	A	Cmax in Wistar rat at 2 mg/kg, po	Rattus norvegicus	3	ALA5126609	organism-based format	Scientific Literature
16.	ALA5130060	A	Tmax in Wistar rat at 2 mg/kg, po	Rattus norvegicus	3	ALA5126609	organism-based format	Scientific Literature
17.	ALA5130061	A	AUC in Balb/c mouse at 10 mg/kg, po	Mus musculus	10	ALA5126609	organism-based format	Scientific Literature
18.	ALA5130063	A	AUC in Wistar rat at 2 mg/kg, po	Rattus norvegicus	5	ALA5126609	organism-based format	Scientific Literature
19.	ALA5130064	A	Oral bioavailability in Wistar rat at 2 mg/kg	Rattus norvegicus	5	ALA5126609	organism-based format	Scientific Literature
20.	ALA5130068	A	Clearance in Wistar rat at 2 mg/kg, po	Rattus norvegicus	3	ALA5126609	organism-based format	Scientific Literature