#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3888750	B	33P-Based Assay: The biochemical EC50 (half-maximal concentration required for full activation) of compounds for the activation of AMPK was evaluated by 33P-based assay using SAMS peptide (commercially available) derived from ACC-1. Twenty μl of phosphorylated AMPK diluted in assay buffer, (50 mM HEPES, 1 mM EGTA, 10 mM MgCl2, 0.25 mM DTT, 0.01% Tween-20, 0.01% BSA (pH 7.5)) was added to 384 well plates containing 1 μL of test compound. Following a fifteen minute room temperature incubation, 10 μL of protein phosphatase PP2A was added to the plate to dephosphorylate pThr172 of AMPK. After incubation for 90 minutes, 10 μL of substrate mixture containing 41 nM okadaic acid, 82 μM SAMS peptide, 82 μM ATP and tracer amounts (6.8 nM) of 33P-containing ATP was added to the plate. The reaction was terminated after 60 minutes incubation at room temperature by the addition of 15 μL of 2% H3PO4. Subsequently, 45 μl of reaction mix was transferred to 384 well Millipore MZPH filter plates (MZPHNO50) pre-treated with 25 μL of 2% H3PO4 and the plates were washed three times with assay buffer. 20 μL of Ready Safe scintillation fluid was added to dried plates followed by detection on the Trilux detector.	Homo sapiens	177	ALA3886819	protein complex format	BindingDB Database
2.	ALA4023942	A	Total plasma clearance in Wistar Han rat at 1 mg/kg, iv measured up to 24 hrs post dose by LC-MS/MS analysis	Rattus norvegicus	7	ALA4020835	organism-based format	Scientific Literature
3.	ALA4023943	P	Dissociation constant, pKa of the compound by capillary electrophoresis		14	ALA4020835	small-molecule physicochemical format	Scientific Literature
4.	ALA4023944	A	Drug excretion in Wistar Han rat urine assessed as compound recovered at 1 mg/kg, iv measured over 24 hrs by LC/MS analysis	Rattus norvegicus	6	ALA4020835	organism-based format	Scientific Literature
5.	ALA4023945	A	Drug excretion in cynomolgus monkey urine assessed as compound recovered at 1 mg/kg, iv measured over 24 hrs by LC/MS analysis	Macaca fascicularis	2	ALA4020835	organism-based format	Scientific Literature
6.	ALA4023961	P	Thermodynamic solubility of the compound in sodium phosphate buffer at pH 7.4 after 24 hrs by nitrogen detection method		2	ALA4020835	small-molecule physicochemical format	Scientific Literature
7.	ALA4023962	B	Allosteric activation of human AMPK alpha1/beta1/gamma1 by TR-FRET assay	Homo sapiens	14	ALA4020835	protein complex format	Scientific Literature
8.	ALA4023963	B	Allosteric activation of human AMPK alpha1/beta1/gamma1 by TR-FRET assay relative to maximum activation of AMP	Homo sapiens	14	ALA4020835	protein complex format	Scientific Literature
9.	ALA4023964	B	Binding affinity to human BAP-tagged AMPK alpha1/beta1/gamma1 by SPR assay	Homo sapiens	14	ALA4020835	protein complex format	Scientific Literature
10.	ALA4023965	P	Lipophilicity, log D of compound at pH 7.4 by shake-flask method		14	ALA4020835	small-molecule physicochemical format	Scientific Literature
11.	ALA4023966	A	Apparent permeability from apical to basolateral in RRCK cells after 1 hr by LC-MS analysis	Canis lupus familiaris	14	ALA4020835	cell-based format	Scientific Literature
12.	ALA4023967	A	Total intrinsic clearance in human hepatocytes assessed as per million cells at 1 uM after 4 hrs by LC-MS/MS analysis	Homo sapiens	14	ALA4020835	cell-based format	Scientific Literature
13.	ALA4023968	A	Substrate activity at human OAT3 expressed in HEK293 cells assessed as ratio of compound uptake in human OAT3 transfected HEK293 cells to uptake in vector-treated HEK293 cells at 1 uM after 3 mins by LC-MS analysis	Homo sapiens	14	ALA4020835	cell-based format	Scientific Literature
14.	ALA4023969	A	Substrate activity at rat OAT3 expressed in HEK293 cells assessed as ratio of compound uptake in rat OAT3 transfected HEK293 cells to uptake in vector-treated HEK293 cells at 1 uM after 3 mins by LC-MS analysis	Rattus norvegicus	3	ALA4020835	cell-based format	Scientific Literature
15.	ALA4023970	A	Substrate activity at human OAT1 expressed in HEK293 cells assessed as ratio of compound uptake in human OAT1 transfected HEK293 cells to uptake in vector-treated HEK293 cells at 1 uM after 3 mins by LC-MS analysis	Homo sapiens	6	ALA4020835	cell-based format	Scientific Literature
16.	ALA4023971	A	Plasma clearance in Wistar Han rat at 1 mg/kg, iv via bolus dose measured up to 24 hrs post dose by LC-MS/MS analysis	Rattus norvegicus	2	ALA4020835	organism-based format	Scientific Literature
17.	ALA4023972	A	Plasma clearance in cynomolgus monkey at 1 mg/kg, iv via bolus dose measured up to 24 hrs post dose by LC-MS/MS analysis	Macaca fascicularis	2	ALA4020835	organism-based format	Scientific Literature
18.	ALA4023973	A	Volume of distribution at steady state in Wistar Han rat at 1 mg/kg, iv via bolus dose measured up to 24 hrs post dose by LC-MS/MS analysis	Rattus norvegicus	2	ALA4020835	organism-based format	Scientific Literature
19.	ALA4023974	A	Volume of distribution at steady state in cynomolgus monkey at 1 mg/kg, iv via bolus dose measured up to 24 hrs post dose by LC-MS/MS analysis	Macaca fascicularis	2	ALA4020835	organism-based format	Scientific Literature
20.	ALA4023975	A	Half life in Wistar Han rat at 1 mg/kg, iv via bolus dose measured up to 24 hrs post dose by LC-MS/MS analysis	Rattus norvegicus	2	ALA4020835	organism-based format	Scientific Literature