# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4009309 | Binding | Activation of BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of interleukin 2 stimulated cell proliferation treated for 72 hrs measured on day 11 post last treatment by flow cytometry | Homo sapiens | 8 | ALA4007426 | assay format | Scientific Literature | |
2. | ALA4009310 | Binding | Potency index, ratio of HMBPP EC50 to compound EC50 for BTN3A1 in human Vgamma9Vdelta2 T cells | Homo sapiens | 7 | ALA4007426 | assay format | Scientific Literature | |
3. | ALA4009313 | Binding | Binding affinity to recombinant C-terminal His-tagged BTN3A1 intracellular domain (unknown origin) expressed in BL21(DE) by isothermal titration calorimetry | Homo sapiens | 1 | ALA4007426 | cell-based format | Scientific Literature | |
4. | ALA4367522 | Binding | Activation of BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of IL2-stimulated cell proliferation by measuring increase in CD3+ cells preincubated for 3 days followed by 11 days incubation post compound washout and subsequent IL2 stimulation for every 3 days measured after 14 days by flow cytometry | Homo sapiens | 11 | ALA4364297 | cell-based format | Scientific Literature | |
5. | ALA4367529 | Binding | Activation of BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of IL2-stimulated IFNgamma production measured after 48 hrs by ELISA | Homo sapiens | 11 | ALA4364297 | assay format | Scientific Literature | |
6. | ALA4367532 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 full interaction domain (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins in presence of calcium by fluorescence polarization assay | Homo sapiens | 5 | ALA4364297 | assay format | Scientific Literature | |
7. | ALA4367534 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 B30.2 domain (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay | Homo sapiens | 11 | ALA4364297 | assay format | Scientific Literature | |
8. | ALA4367538 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 W421A mutant (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA4364297 | assay format | Scientific Literature | |
9. | ALA4367539 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 M424A mutant (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA4364297 | assay format | Scientific Literature | |
10. | ALA4367540 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 R442A mutant (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA4364297 | assay format | Scientific Literature | |
11. | ALA4367541 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 R448A mutant (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA4364297 | assay format | Scientific Literature | |
12. | ALA4367542 | Binding | Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 R499A mutant (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA4364297 | assay format | Scientific Literature | |
13. | ALA5030445 | Binding | Displacement of GDP-BODIPY probe from BTN3A1 full intracellular domain (unknown origin) at 10 mM measured after 60 mins by fluorescence polarization assay | Homo sapiens | 3 | ALA5029197 | cell-based format | Scientific Literature |