#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4367522	Binding	Activation of BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of IL2-stimulated cell proliferation by measuring increase in CD3+ cells preincubated for 3 days followed by 11 days incubation post compound washout and subsequent IL2 stimulation for every 3 days measured after 14 days by flow cytometry	Homo sapiens	11	ALA4364297	cell-based format	Scientific Literature
2.	ALA4367529	Binding	Activation of BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of IL2-stimulated IFNgamma production measured after 48 hrs by ELISA	Homo sapiens	11	ALA4364297	assay format	Scientific Literature
3.	ALA4367534	Binding	Displacement of GDP-BODIPY from C-terminal 6His-tagged BTN3A1 B30.2 domain (unknown origin) expressed in Escherichia coli BL21 (DE) measured after 60 mins by fluorescence polarization assay	Homo sapiens	11	ALA4364297	assay format	Scientific Literature
4.	ALA4009307	Binding	Activation of BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of interleukin 2 stimulated cell proliferation by measuring increase in CD3 positive cells at 0.1 uM treated for 72 hrs measured on day 11 post last treatment by flow cytometry	Homo sapiens	3	ALA4007426	cell-based format	Scientific Literature
5.	ALA4009308	Binding	Activation of BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of interleukin 2 stimulated cell proliferation by measuring increase in gammadelta T cell receptor positive cells at 0.1 uM treated for 72 hrs measured on day 11 post last treatment by flow cytometry	Homo sapiens	3	ALA4007426	assay format	Scientific Literature
6.	ALA4009309	Binding	Activation of BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of interleukin 2 stimulated cell proliferation treated for 72 hrs measured on day 11 post last treatment by flow cytometry	Homo sapiens	8	ALA4007426	assay format	Scientific Literature
7.	ALA4009310	Binding	Potency index, ratio of HMBPP EC50 to compound EC50 for BTN3A1 in human Vgamma9Vdelta2 T cells	Homo sapiens	7	ALA4007426	assay format	Scientific Literature
8.	ALA4009311	Toxicity	Toxicity in human Vgamma9/Vdelta2 T cells at >= 10 uM treated for 72 hrs measured on day 11 post last treatment by flow cytometry	Homo sapiens	1	ALA4007426	cell-based format	Scientific Literature
9.	ALA4009312	Toxicity	Toxicity in human Vgamma9/Vdelta2 T cells treated for 72 hrs measured on day 11 post last treatment by flow cytometry	Homo sapiens	7	ALA4007426	cell-based format	Scientific Literature
10.	ALA4009323	Binding	Binding affinity to recombinant C-terminal His-tagged BTN3A1 intracellular domain (unknown origin) expressed in BL21(DE) at 240 to 860 uM by isothermal titration calorimetry	Homo sapiens	1	ALA4007426	cell-based format	Scientific Literature
11.	ALA4009329	Functional	Selectivity index, ratio of IC50 for human Vgamma9Vdelta2 T cells to EC50 for BTN3A1 in human Vgamma9Vdelta2 T cells	Homo sapiens	4	ALA4007426	assay format	Scientific Literature
12.	ALA4009330	Binding	Activation of BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of T effector cells mediated K562 cell lysis preincubated with K562 cells for 2 hrs followed by T effector cell addition measured after 4 hrs	Homo sapiens	6	ALA4007426	assay format	Scientific Literature
13.	ALA4009331	Binding	Potency index, ratio of HMBPP EC50 to compound EC50 for BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of T effector cells mediated K562 cell lysis	Homo sapiens	5	ALA4007426	assay format	Scientific Literature
14.	ALA4009332	ADME	Antiproliferative activity against human K562 cells after 72 hrs by fluorescence spectroscopy	Homo sapiens	6	ALA4007426	cell-based format	Scientific Literature
15.	ALA4009333	ADME	Safety index, ratio of HMBPP IC50 to compound IC50 for human K562 cells	Homo sapiens	2	ALA4007426	cell-based format	Scientific Literature