# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3999523 | F | Antioxidant activity in FRDA patient derived lymphocytes assessed as inhibition of DEM-mediated lipid peroxidation at 0.1 to 2.5 uM pretreated for 16 hrs followed by DEM addition measured after 120 mins by C11-BODIPY581/591 probe-based FACS analysis | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
2. | ALA3999524 | F | Antioxidant activity in FRDA patient derived lymphocytes assessed as inhibition of DEM-mediated reactive oxygen species formation at 0.1 to 2.5 uM pretreated for 16 hrs followed by DEM addition measured after 80 mins by DCFH-DA dye-based flow cytometric analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
3. | ALA3999526 | F | Cytoprotective activity in DEM-treated FRDA patient derived lymphocytes assessed as preservation of mitochondrial inner membrane potential at 2.5 uM pretreated for 16 hrs followed by DEM addition measured after 120 mins by TMRM dye-based flow cytometric analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
4. | ALA3999528 | F | Cytoprotective activity against DEM-induced oxidative stress in FRDA patient derived lymphocytes assessed as increase in cell viability at 0.1 to 2.5 uM pretreated for 16 hrs followed by DEM addition measured after 6 hrs by calcein acetoxy-methyl-ester/ethidium homodimer-1 staining-based flow cytometric analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
5. | ALA3999530 | F | Cytoprotective activity in FRDA patient derived lymphocytes cultured in glucose-free media supplemented with galactose assessed as total intracellular ATP levels at 1 uM after 48 hrs by luciferin-luciferase reaction-based bioluminescence assay relative to control | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
6. | ALA3999531 | F | Cytoprotective activity in FRDA patient derived lymphocytes cultured in glucose-free media supplemented with galactose assessed as total intracellular ATP levels at 5 uM after 48 hrs by luciferin-luciferase reaction-based bioluminescence assay relative to control | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
7. | ALA3999532 | F | Cytoprotective activity in FRDA patient derived lymphocytes cultured in glucose-free media supplemented with galactose assessed as total intracellular ATP levels at 20 uM after 48 hrs by luciferin-luciferase reaction-based bioluminescence assay relative to control | Homo sapiens | 12 | cell-based format | Scientific Literature | ||
8. | ALA3999534 | A | Metabolic stability in bovine liver microsomes assessed as compound recovery at 25 uM after 30 mins in presence of beta-NADPH by HPLC method | Bos taurus | 9 | assay format | Scientific Literature |