| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4040229 | A | Drug metabolism assessed as recombinant human NEP-medited peptide proteolysis at 1 mM up to 72 hrs by LC-MS analysis | Homo sapiens | 16 | single protein format | Scientific Literature | ||
| 2. | ALA4040230 | A | Drug degradation in human plasma assessed as NEP-mediated degradation by measuring amount of apelin remaining after 30 mins by RP-HPLC analysis | Homo sapiens | 16 | single protein format | Scientific Literature | ||
| 3. | ALA4040231 | A | Drug degradation in human plasma assessed as NEP-mediated degradation by measuring amount of apelin remaining after 60 mins by RP-HPLC analysis | Homo sapiens | 16 | single protein format | Scientific Literature | ||
| 4. | ALA4040232 | A | Inhibition of recombinant human NEP using pyr-1-apelin-13 as substrate assessed as peptide degradation at 1 mM preincubated for 10 mins followed by substrate addition measured after 72 hrs by LC-MS analysis | Homo sapiens | 12 | single protein format | Scientific Literature | ||
| 5. | ALA4040233 | B | Displacement of [125I]-pyr-1-apelin-13 from rat C-terminal EGFP-tagged APJ receptor expressed in CHO cell membranes after 3 hrs by Wallac gamma counting | Rattus norvegicus | 16 | cell-based format | Scientific Literature | ||
| 6. | ALA4040235 | F | Cardiovascular activity in anesthetized mouse assessed as decrease in blood pressure at 1.4 uM/kg, iv relative to control | Mus musculus | 8 | organism-based format | Scientific Literature | ||
| 7. | ALA4040236 | F | Cardioprotective activity against Langendorff isolated mouse heart ischemia-reperfusion model assessed as maximum derivative change in systolic pressure over time at 1 uM administered for 10 mins during start of 40 mins reperfusion period post 30 mins ischemic induction | Mus musculus | 5 | tissue-based format | Scientific Literature | ||
| 8. | ALA4040237 | F | Cardioprotective activity against Langendorff isolated mouse heart ischemia-reperfusion model assessed as improvement in LVDP at 1 uM administered for 10 mins during start of 40 mins reperfusion period post 30 mins ischemic induction | Mus musculus | 5 | tissue-based format | Scientific Literature | ||
| 9. | ALA4040238 | F | Cardioprotective activity against Langendorff isolated mouse heart ischemia-reperfusion model assessed as improvement in RPP at 1 uM administered for 10 mins during start of 40 mins reperfusion period post 30 mins ischemic induction | Mus musculus | 5 | tissue-based format | Scientific Literature | ||
| 10. | ALA4040239 | F | Cardioprotective activity against Langendorff isolated mouse heart ischemia-reperfusion model assessed as minimum derivative change in diastolic pressure over time at 1 uM administered for 10 mins during start of 40 mins reperfusion period post 30 mins ischemic induction | Mus musculus | 5 | tissue-based format | Scientific Literature | ||
| 11. | ALA4040240 | T | Cardiotoxicity in anesthetized mouse assessed as induction of tachycardia at 1.4 uM/kg, iv relative to control | Mus musculus | 8 | organism-based format | Scientific Literature | ||
| 12. | ALA4401034 | A | Substrate activity at recombinant human C-terminal His6-tagged KLKB1 (Pro19 to Ser262 residues) expressed in mammalian expression system assessed as Km measured after 30 to 60 mins by LC-MS analysis | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 13. | ALA4401035 | A | Substrate activity at recombinant human C-terminal His6-tagged KLKB1 (Pro19 to Ser262 residues) expressed in mammalian expression system assessed as Kcat measured after 30 to 60 mins by LC-MS analysis | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 14. | ALA4401036 | A | Substrate activity at recombinant human C-terminal His6-tagged KLKB1 (Pro19 to Ser262 residues) expressed in mammalian expression system assessed as ratio of Kcat to Km measured after 30 to 60 mins by LC-MS analysis | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 15. | ALA4401043 | A | Substrate activity at recombinant human C-terminal His6-tagged KLKB1 (Pro19 to Ser262 residues) expressed in mammalian expression system assessed as Vmax measured after 30 to 60 mins by LC-MS analysis | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 16. | ALA4401044 | A | Substrate activity at recombinant human C-terminal His6-tagged KLKB1 (Pro19 to Ser262 residues) expressed in mammalian expression system assessed as compound half life by LC-MS analysis | Homo sapiens | 7 | assay format | Scientific Literature | ||
| 17. | ALA4401045 | A | Substrate activity at recombinant human NEP (Tyr52 to Trp750 residues) expressed in HEK293 cells assessed as compound half life by LC-MS analysis | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 18. | ALA4401046 | A | Substrate activity at KLKB1 in human plasma assessed as compound half life by LC-MS analysis | Homo sapiens | 7 | cell-free format | Scientific Literature | ||
| 19. | ALA4401047 | B | Displacement of [125I]-(Glp1,Nle11,Try14)apelin-13 from recombinant rat EGFP-fused apelin receptor expressed in CHO cell membrane measured after 3 hrs | Rattus norvegicus | 9 | cell-based format | Scientific Literature | ||
| 20. | ALA4401048 | F | Activation of recombinant human APJ expressed in rat Chem5 cells co-expressing Galpha15 by Calcium 6-QF dye based fluorescence assay | Homo sapiens | 9 | cell-based format | Scientific Literature |
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