| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4028398 | B | Inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) using nicotinamide/SAM as substrate/co-factor measured for 20 mins by SAHH-coupled fluorescent assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 2. | ALA4028399 | B | Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) by isothermal titration calorimetric method | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 3. | ALA4028400 | B | Inhibition of DOT1L (unknown origin) after 1 hr by filter-based assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA4028401 | B | Inhibition of PRMT7 (unknown origin) using [3H]-SAM as co-factor by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA4028402 | B | Inhibition of BCDIN3D (unknown origin) using [3H]-SAM as co-factor by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA4028403 | B | Inhibition of SMYD2 (unknown origin) using [3H]-SAM as co-factor by scintillation proximity assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA4028404 | B | Competitive inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) at 1 to 250 uM measured for 20 mins in presence of varying concentration of SAM by Lineweaver-Burk plot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA4028405 | B | Nonompetitive inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) at 1 to 250 uM measured for 20 mins in presence of varying concentration of nicotinamide by Lineweaver-Burk plot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA4028406 | B | Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in enthalpy by isothermal titration calorimetric method | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 10. | ALA4028407 | B | Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in entropy by isothermal titration calorimetric method | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 11. | ALA4306964 | B | Inhibition of wild-type human full length NNMT expressed in Escherichia coli BL21(DE3) cells assessed as reduction in 1-methyl-nicotinamide formation pre-incubated for 10 mins followed by AdoMet and nicotinamide addition measured after 30 mins by UHP-HILIC/Q-TOF-MS analysis | Homo sapiens | 29 | cell-based format | Scientific Literature | ||
| 12. | ALA4306965 | B | Inhibition of wild-type human full length NNMT expressed in Escherichia coli BL21(DE3) cells assessed as reduction in 1-methyl-nicotinamide formation at 250 uM pre-incubated for 10 mins followed by AdoMet and nicotinamide addition measured after 30 mins by UHP-HILIC/Q-TOF-MS analysis relative to control | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
| 13. | ALA4306974 | F | Antiproliferative activity against human HSC2 cells assessed as reduction in cell viability at 1 to 100 uM measured up to 72 hrs by MTT assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 14. | ALA4416333 | B | Inhibition of N-terminal His6-tagged wild type human NNMT expressed in Escherichia coli NiCo21(DE3) assessed as reduction in 1-methylquinolinium level using quinoline as substrate in presence of SAM and measured every 27 seconds for 5.5 mins by fluorescence-based assay | Homo sapiens | 35 | assay format | Scientific Literature |
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