#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4028398	B	Inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) using nicotinamide/SAM as substrate/co-factor measured for 20 mins by SAHH-coupled fluorescent assay	Homo sapiens	2	ALA4024794	assay format	Scientific Literature
2.	ALA4028399	B	Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) by isothermal titration calorimetric method	Homo sapiens	2	ALA4024794	assay format	Scientific Literature
3.	ALA4028400	B	Inhibition of DOT1L (unknown origin) after 1 hr by filter-based assay	Homo sapiens	1	ALA4024794	single protein format	Scientific Literature
4.	ALA4028401	B	Inhibition of PRMT7 (unknown origin) using [3H]-SAM as co-factor by scintillation proximity assay	Homo sapiens	1	ALA4024794	single protein format	Scientific Literature
5.	ALA4028402	B	Inhibition of BCDIN3D (unknown origin) using [3H]-SAM as co-factor by scintillation proximity assay	Homo sapiens	1	ALA4024794	single protein format	Scientific Literature
6.	ALA4028403	B	Inhibition of SMYD2 (unknown origin) using [3H]-SAM as co-factor by scintillation proximity assay	Homo sapiens	1	ALA4024794	single protein format	Scientific Literature
7.	ALA4028404	B	Competitive inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) at 1 to 250 uM measured for 20 mins in presence of varying concentration of SAM by Lineweaver-Burk plot analysis	Homo sapiens	1	ALA4024794	assay format	Scientific Literature
8.	ALA4028405	B	Nonompetitive inhibition of human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) at 1 to 250 uM measured for 20 mins in presence of varying concentration of nicotinamide by Lineweaver-Burk plot analysis	Homo sapiens	1	ALA4024794	assay format	Scientific Literature
9.	ALA4028406	B	Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in enthalpy by isothermal titration calorimetric method	Homo sapiens	2	ALA4024794	assay format	Scientific Literature
10.	ALA4028407	B	Binding affinity to human NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in entropy by isothermal titration calorimetric method	Homo sapiens	2	ALA4024794	assay format	Scientific Literature
11.	ALA4306964	B	Inhibition of wild-type human full length NNMT expressed in Escherichia coli BL21(DE3) cells assessed as reduction in 1-methyl-nicotinamide formation pre-incubated for 10 mins followed by AdoMet and nicotinamide addition measured after 30 mins by UHP-HILIC/Q-TOF-MS analysis	Homo sapiens	29	ALA4304774	cell-based format	Scientific Literature
12.	ALA4306965	B	Inhibition of wild-type human full length NNMT expressed in Escherichia coli BL21(DE3) cells assessed as reduction in 1-methyl-nicotinamide formation at 250 uM pre-incubated for 10 mins followed by AdoMet and nicotinamide addition measured after 30 mins by UHP-HILIC/Q-TOF-MS analysis relative to control	Homo sapiens	11	ALA4304774	cell-based format	Scientific Literature
13.	ALA4306974	F	Antiproliferative activity against human HSC2 cells assessed as reduction in cell viability at 1 to 100 uM measured up to 72 hrs by MTT assay	Homo sapiens	3	ALA4304774	cell-based format	Scientific Literature
14.	ALA4416333	B	Inhibition of N-terminal His6-tagged wild type human NNMT expressed in Escherichia coli NiCo21(DE3) assessed as reduction in 1-methylquinolinium level using quinoline as substrate in presence of SAM and measured every 27 seconds for 5.5 mins by fluorescence-based assay	Homo sapiens	35	ALA4414609	assay format	Scientific Literature